Cox-2 Antibody (D-12): sc-166475
- Cox-2 Antibody (D-12) is a mouse monoclonal IgG1 κ, cited in 68 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 586-604 at the C-terminus of Cox-2 of mouse origin
- Anti-Cox-2 Antibody (D-12) is recommended for detection of Cox-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Cox-2 Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cox-2 Antibody (D-12) for WB and IHC(P) applications. These reagents are now offered in bundles with Cox-2 Antibody (D-12) (see ordering information below).
Cox-2 Antibody (D-12) is a mouse monoclonal IgG1 kappa light chain antibody specific for an epitope mapping between amino acids 586-604 at the C-terminus of Cyclooxygenase-2 (Cox-2) of mouse origin. Cox-2 (D-12) antibody reacts with mouse, rat, and human proteins and is suitable for western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry with paraffin-embedded sections (IHCP). Cox-2, also known as Prostaglandin-Endoperoxide Synthase 2 (PTGS2), is an inducible enzyme located primarily in the endoplasmic reticulum and nuclear envelope within cells, which is crucial for prostaglandin biosynthesis. Cox-2′s cellular localization allows efficient interaction with membrane-bound substrates and access to signaling pathways that regulate inflammation and pain responses. Unlike constitutively expressed Cox-1, Cox-2 shows minimal expression under normal physiological conditions but significantly upregulates in response to inflammatory stimuli, cytokines, and growth factors. Such upregulation leads to increased production of prostaglandins, contributing to inflammation, pain, and fever. Cox-2 overexpression has been implicated in various pathological conditions, including chronic inflammation and progression of certain cancers such as colorectal, breast, and lung cancer. Cox-2 serves as a key target for nonsteroidal anti-inflammatory drugs (NSAIDs) and selective Cox-2 inhibitors, which aim to reduce inflammation and pain by inhibiting prostaglandin synthesis. Understanding Cox-2′s cellular localization and function is essential for elucidating mechanisms underlying inflammatory diseases and cancer progression, thereby aiding in developing targeted therapeutic strategies. Anti-Cox-2 antibody (D-12) serves as a valuable tool for researchers investigating Cox-2 expression, localization, and role in various physiological and pathological processes.
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LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cox-2 Antibody (D-12) | sc-166475 | 200 µg/ml | $316.00 | |||
Cox-2 Antibody (D-12): m-IgG Fc BP-HRP Bundle | sc-528975 | 200 µg Ab; 10 µg BP | $354.00 | |||
Cox-2 Antibody (D-12): m-IgGκ BP-HRP Bundle | sc-521623 | 200 µg Ab, 40 µg BP | $354.00 | |||
Cox-2 Antibody (D-12): m-IgG1 BP-HRP Bundle | sc-543248 | 200 µg Ab; 20 µg BP | $354.00 | |||
Cox-2 Antibody (D-12) AC | sc-166475 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Cox-2 Antibody (D-12) HRP | sc-166475 HRP | 200 µg/ml | $316.00 | |||
Cox-2 Antibody (D-12) FITC | sc-166475 FITC | 200 µg/ml | $330.00 | |||
Cox-2 Antibody (D-12) PE | sc-166475 PE | 200 µg/ml | $343.00 | |||
Cox-2 Antibody (D-12) Alexa Fluor® 488 | sc-166475 AF488 | 200 µg/ml | $357.00 | |||
Cox-2 Antibody (D-12) Alexa Fluor® 546 | sc-166475 AF546 | 200 µg/ml | $357.00 | |||
Cox-2 Antibody (D-12) Alexa Fluor® 594 | sc-166475 AF594 | 200 µg/ml | $357.00 | |||
Cox-2 Antibody (D-12) Alexa Fluor® 647 | sc-166475 AF647 | 200 µg/ml | $357.00 | |||
Cox-2 Antibody (D-12) Alexa Fluor® 680 | sc-166475 AF680 | 200 µg/ml | $357.00 | |||
Cox-2 Antibody (D-12) Alexa Fluor® 790 | sc-166475 AF790 | 200 µg/ml | $357.00 | |||
Cox-2 (D-12) Neutralizing Peptide | sc-166475 P | 100 µg/0.5 ml | $68.00 |