Cox-2 Antibody (29): sc-19999

Cox-2 Antibody (29) is a mouse monoclonal IgG1 kappa light chain antibody raised against a C-terminal peptide consisting of amino acids 580-598 of human Cyclooxygenase-2 (Cox-2), reactive with mouse, rat, and human proteins, and suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and immunohistochemistry with paraffin-embedded sections (IHCP). Cox-2, also known as Prostaglandin-Endoperoxide Synthase 2 (PTGS2), is an inducible enzyme that plays a pivotal role in the conversion of arachidonic acid to prostaglandins, which are lipid mediators involved in inflammation, pain, and fever. Unlike its constitutively expressed isoform Cox-1, Cox-2 is minimally expressed under normal physiological conditions but is significantly upregulated in response to inflammatory stimuli, cytokines, growth factors, and tumor promoters. This upregulation leads to increased production of prostaglandins, contributing to the inflammatory response and pain perception. Overexpression of Cox-2 has been implicated in various pathological conditions, including chronic inflammation, arthritis, and the progression of certain cancers such as colorectal, breast, and lung cancer. Cox-2 is a key therapeutic target for nonsteroidal anti-inflammatory drugs (NSAIDs) and selective Cox-2 inhibitors (coxibs), which aim to reduce inflammation and pain by inhibiting prostaglandin synthesis. Understanding the expression and regulation of Cox-2 is crucial for elucidating the mechanisms underlying inflammatory diseases and cancer progression, thereby aiding in the development of targeted therapeutic strategies.

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Cox-2 Antibody (29) References:

1. Overexpression of human prostaglandin G/H synthase-1 and -2 by recombinant vaccinia virus: inhibition by nonsteroidal anti-inflammatory drugs and biosynthesis of 15-hydroxyeicosatetraenoic acid.  |  O'Neill, GP., et al. 1994. Mol Pharmacol. 45: 245-54. PMID: 8114674
2. Expression of mRNA for cyclooxygenase-1 and cyclooxygenase-2 in human tissues.  |  O'Neill, GP. and Ford-Hutchinson, AW. 1993. FEBS Lett. 330: 156-60. PMID: 8365485
3. Prostaglandin synthase 2 gene disruption causes severe renal pathology in the mouse.  |  Morham, SG., et al. 1995. Cell. 83: 473-82. PMID: 8521477
4. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.  |  Langenbach, R., et al. 1995. Cell. 83: 483-92. PMID: 8521478
5. Alterations in cellular adhesion and apoptosis in epithelial cells overexpressing prostaglandin endoperoxide synthase 2.  |  Tsujii, M. and DuBois, RN. 1995. Cell. 83: 493-501. PMID: 8521479
6. Cyclooxygenase-2 induction in cerebral cortex: an intracellular response to synaptic excitation.  |  Adams, J., et al. 1996. J Neurochem. 66: 6-13. PMID: 8522990
7. Synergistic effect of interleukin-1 beta and tumor necrosis factor alpha on PGE2 production by articular chondrocytes does not involve PLA2 stimulation.  |  Berenbaum, F., et al. 1996. Exp Cell Res. 222: 379-84. PMID: 8598226

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cox-2 Antibody (29) has 241 citations in a variety of scientific publications.

Primary antibodies like Anti-Cox-2 Antibody (29) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cox-2 Antibody (29) Specifications

Alternate Product Name: Cox-2

Product Clone Name: 29

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 580-598

Epitope Target: Cox-2

Epitope Species: human

Product Reactivity/Detection: Cox-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 241 citations in various scientific publications

Available Conjugates/Forms for Cox-2 Antibody (29): AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin

The preferred Cox-2 antibody is Cox-2 Antibody (H-3): sc-376861.