Cortactin Antibody (A-4): sc-55578
- Cortactin Antibody (A-4) is a mouse monoclonal IgG1 κ, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 309-499 of Cortactin of human origin
- Anti-Cortactin Antibody (A-4) is recommended for detection of Cortactin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Cortactin Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cortactin Antibody (A-4) for WB and IHC(P) applications. These reagents are now offered in bundles with Cortactin Antibody (A-4) (see ordering information below).
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SEE ALSO...
Cortactin Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody specifically engineered to recognize Cortactin, also known as Ems-1, a vital filamentous actin (F-actin) binding protein that plays a key role in the regulation of the cytoskeleton, influencing essential cellular processes such as motility and shape maintenance. Anti-Cortactin antibody (A-4) is raised against amino acids 309-499 of human Cortactin and demonstrates robust species reactivity, effectively detecting Cortactin from mouse, rat, and human origins across a variety of applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Cortactin functions as a substrate for Src p60 kinase, where its tyrosine phosphorylation by Src p60 modulates its binding affinity to F-actin and its ability to cross-link actin filaments, thereby impacting cellular structural integrity and dynamics. Structurally, Cortactin features tandem 37 amino acid repeats at the N-terminus and an SH3 domain at the C-terminus, which are crucial for its interaction with actin filaments and various signaling molecules. Additionally, Cortactin is phosphorylated in response to growth factors such as FGF-1, highlighting its involvement in signal transduction pathways. Cortactin is abundantly expressed in megakaryocytes and platelets, suggesting a significant role in the maturation and function of these cells. Cortactin (A-4) antibody is available in multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing researchers with versatile options to suit diverse experimental needs. Anti-Cortactin antibody (A-4) is an indispensable tool for scientists investigating cytoskeletal dynamics, cell signaling mechanisms, and hematopoietic processes, ensuring accurate and reliable detection of Cortactin in their studies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cortactin Antibody (A-4) | sc-55578 | 200 µg/ml | $322.00 | |||
Cortactin Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-526867 | 200 µg Ab; 10 µg BP | $361.00 | |||
Cortactin Antibody (A-4): m-IgG1 BP-HRP Bundle | sc-532240 | 200 µg Ab; 20 µg BP | $361.00 | |||
Cortactin Antibody (A-4) AC | sc-55578 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Cortactin Antibody (A-4) HRP | sc-55578 HRP | 200 µg/ml | $322.00 | |||
Cortactin Antibody (A-4) FITC | sc-55578 FITC | 200 µg/ml | $336.00 | |||
Cortactin Antibody (A-4) PE | sc-55578 PE | 200 µg/ml | $349.00 | |||
Cortactin Antibody (A-4) Alexa Fluor® 488 | sc-55578 AF488 | 200 µg/ml | $364.00 | |||
Cortactin Antibody (A-4) Alexa Fluor® 546 | sc-55578 AF546 | 200 µg/ml | $364.00 | |||
Cortactin Antibody (A-4) Alexa Fluor® 594 | sc-55578 AF594 | 200 µg/ml | $364.00 | |||
Cortactin Antibody (A-4) Alexa Fluor® 647 | sc-55578 AF647 | 200 µg/ml | $364.00 | |||
Cortactin Antibody (A-4) Alexa Fluor® 680 | sc-55578 AF680 | 200 µg/ml | $364.00 | |||
Cortactin Antibody (A-4) Alexa Fluor® 790 | sc-55578 AF790 | 200 µg/ml | $364.00 |