CoREST Antibody (H-8): sc-376567

CoREST Antibody (H-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects CoREST protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CoREST (H-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CoREST protein plays a crucial role in gene expression regulation, particularly in neuronal development, by functioning as a co-repressor in the neuron-restrictive silencer factor (NRSF) complex. This complex maintains neuronal gene silencing in non-neuronal cells, preventing inappropriate expression that could disrupt cellular function. CoREST′s interaction with NRSF and other co-repressors, such as Sin3A, is vital for recruiting histone deacetylases, which modify chromatin structure to repress transcription. CoREST′s structural integrity, including its SANT domain that facilitates interaction with histone deacetylases, underscores its importance in epigenetic regulation. CoREST functionality has been conserved across various species, including humans, mice, Xenopus, and Caenorhabditis elegans, highlighting its fundamental role in gene regulation and cellular differentiation.

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CoREST Antibody (H-8) References:

1. CoREST: a functional corepressor required for regulation of neural-specific gene expression.  |  Andrés, ME., et al. 1999. Proc Natl Acad Sci U S A. 96: 9873-8. PMID: 10449787
2. The co-repressor mSin3A is a functional component of the REST-CoREST repressor complex.  |  Grimes, JA., et al. 2000. J Biol Chem. 275: 9461-7. PMID: 10734093
3. CoREST is an integral component of the CoREST- human histone deacetylase complex.  |  You, A., et al. 2001. Proc Natl Acad Sci U S A. 98: 1454-8. PMID: 11171972
4. Cell-type non-selective transcription of mouse and human genes encoding neural-restrictive silencer factor.  |  Kojima, T., et al. 2001. Brain Res Mol Brain Res. 90: 174-86. PMID: 11406295
5. Identification and localization of M-CoREST (1A13), a mouse homologue of the human transcriptional co-repressor CoREST, in the developing mouse CNS.  |  Tontsch, S., et al. 2001. Mech Dev. 108: 165-9. PMID: 11578870
6. The Xiro-repressed gene CoREST is expressed in Xenopus neural territories.  |  de la Calle-Mustienes, E., et al. 2002. Mech Dev. 110: 209-11. PMID: 11744385
7. A core-BRAF35 complex containing histone deacetylase mediates repression of neuronal-specific genes.  |  Hakimi, MA., et al. 2002. Proc Natl Acad Sci U S A. 99: 7420-5. PMID: 12032298
8. REST repression of neuronal genes requires components of the hSWI.SNF complex.  |  Battaglioli, E., et al. 2002. J Biol Chem. 277: 41038-45. PMID: 12192000
9. Suppressors of the egg-laying defective phenotype of sel-12 presenilin mutants implicate the CoREST corepressor complex in LIN-12/Notch signaling in C. elegans.  |  Jarriault, S. and Greenwald, I. 2002. Genes Dev. 16: 2713-28. PMID: 12381669
10. Corepressor-dependent silencing of chromosomal regions encoding neuronal genes.  |  Lunyak, VV., et al. 2002. Science. 298: 1747-52. PMID: 12399542
11. Disruption of HDAC/CoREST/REST repressor by dnREST reduces genome silencing and increases virulence of herpes simplex virus.  |  Du, T., et al. 2010. Proc Natl Acad Sci U S A. 107: 15904-9. PMID: 20798038
12. The CoREST repressor complex mediates phenotype switching and therapy resistance in melanoma.  |  Wu, M., et al. 2024. J Clin Invest. 134: PMID: 38300709

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CoREST Antibody (H-8) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-CoREST Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CoREST Antibody (H-8) Specifications

Alternate Product Name: CoREST

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 431-469

Epitope Target: CoREST

Epitope Species: human

Product Reactivity/Detection: CoREST

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for CoREST Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin