Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
copine 1 CRISPR/Cas9 KO Plasmid (h): sc-406621
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- copine 1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the copine 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: copine 1 Antibody (Z6): sc-101269
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
copine 1 CRISPR/Cas9 KO Plasmid (h) | sc-406621 | 20 µg | $397.00 |
Overview
CPNE1 encodes copine 1, a calcium-dependent phospholipid-binding protein containing C2 domains and an A domain that supports membrane recruitment and protein–protein interactions in response to Ca²⁺ signals. Copine 1 has been implicated in coordinating membrane trafficking, cytoskeletal remodeling, and signal transduction by coupling calcium flux to spatially restricted interactions at the plasma membrane and endomembrane compartments. Reported functional links include modulation of receptor-linked signaling and cellular stress responses, with downstream effects on proliferation, migration, and differentiation programs. Dysregulated CPNE1 expression or activity has been associated with tumor biology and other disorders where altered calcium signaling and membrane dynamics contribute to pathogenic phenotypes, making it relevant for mechanistic studies in disease models.
copine 1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CPNE1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CPNE1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CPNE1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish copine 1 protein expression.
This CRISPR knockout system enables efficient generation of CPNE1-deficient cell models for investigation of copine 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CPNE1 exon(s) critical for copine 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CPNE1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by copine 1 CRISPR/Cas9 KO Plasmid (h) and copine 1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CPNE1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by copine 1 HDR Plasmid (h) and copine 1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CPNE1 homology arms to support homology-directed repair at defined CPNE1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.