Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
COPG CRISPR/Cas9 KO Plasmid (h): sc-404407
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COPG CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the COPG genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: COPG Antibody (A-10): sc-393977
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COPG CRISPR/Cas9 KO Plasmid (h) | sc-404407 | 20 µg | $397.00 |
Overview
COPG1 encodes the gamma subunit of coatomer protein complex I (COPI), a cytosolic coat complex that mediates vesicle budding and cargo selection in retrograde transport from the Golgi to the endoplasmic reticulum and within Golgi cisternae. COPG participates in coat assembly with other COPI subunits to regulate membrane trafficking, Golgi organization, and retrieval of ER-resident proteins via sorting signals, thereby supporting protein maturation and secretory pathway homeostasis. Disruption of COPI-dependent trafficking can perturb ER stress responses, glycosylation, and proteostasis, linking COPG1 function to pathways relevant to cell growth, differentiation, and stress adaptation. Altered secretory and trafficking programs are frequently leveraged by proliferative and immune-related disease states, making COPG1 a useful node for mechanistic studies of membrane transport and organelle dynamics.
COPG CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the COPG1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the COPG1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the COPG1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish COPG protein expression.
This CRISPR knockout system enables efficient generation of COPG1-deficient cell models for investigation of COPG signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting COPG1 exon(s) critical for COPG function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple COPG1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by COPG CRISPR/Cas9 KO Plasmid (h) and COPG CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the COPG1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by COPG HDR Plasmid (h) and COPG HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by COPG1 homology arms to support homology-directed repair at defined COPG1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.