COPG Antibody (H-4): sc-271362
- COPG Antibody (H-4) is a mouse monoclonal IgG1 κ COPG antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 575-874 mapping at the C-terminus of COPG of human origin
- COPG Antibody (H-4) is recommended for detection of COPG and γ2-COP of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-COPG Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for COPG Antibody (H-4) for WB applications. These reagents are now offered in bundles with COPG Antibody (H-4) (see ordering information below).
COPG Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects COPG protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-COPG antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. COPG protein, also known as γ-COP, plays a crucial role in forming coat protein complex I (COPI) vesicles, which are essential for retrograde transport from Golgi apparatus back to endoplasmic reticulum (ER) and for intra-Golgi transport. This retrograde transport maintains cellular homeostasis and ensures proteins are correctly processed and recycled within cells. COPG, as part of heptameric coatomer complex, interacts with dilysine motifs on membrane proteins, facilitating their retrieval from Golgi. COPG exhibits highest expression levels in mouse testis and is expressed in a parent-of-origin-specific manner in mammals, highlighting potential significance in reproductive biology and cellular differentiation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
COPG Antibody (H-4) References:
- Duplication of genes encoding non-clathrin coat protein gamma-COP in vertebrate, insect and plant evolution. | Hahn, Y., et al. 2000. FEBS Lett. 482: 31-6. PMID: 11018518
- Identification and characterization of novel isoforms of COP I subunits. | Futatsumori, M., et al. 2000. J Biochem. 128: 793-801. PMID: 11056392
- Interaction of gamma-COP with a transport motif in the D1 receptor C-terminus. | Bermak, JC., et al. 2002. Eur J Cell Biol. 81: 77-85. PMID: 11893085
- Gamma-COP, a coat subunit of non-clathrin-coated vesicles with homology to Sec21p. | Stenbeck, G., et al. 1992. FEBS Lett. 314: 195-8. PMID: 1360908
- Gamma-COP appendage domain - structure and function. | Watson, PJ., et al. 2004. Traffic. 5: 79-88. PMID: 14690497
- In vitro assembly and disassembly of coatomer. | Lowe, M. and Kreis, TE. 1995. J Biol Chem. 270: 31364-71. PMID: 8537409
- A single binding site for dilysine retrieval motifs and p23 within the gamma subunit of coatomer. | Harter, C. and Wieland, FT. 1998. Proc Natl Acad Sci U S A. 95: 11649-54. PMID: 9751720
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COPG Antibody (H-4) | sc-271362 | 200 µg/ml | $316.00 | |||
COPG Antibody (H-4): m-IgG Fc BP-HRP Bundle | sc-527237 | 200 µg Ab; 10 µg BP | $354.00 | |||
COPG Antibody (H-4): m-IgG1 BP-HRP Bundle | sc-532610 | 200 µg Ab; 20 µg BP | $354.00 | |||
COPG Antibody (H-4) AC | sc-271362 AC | 500 µg/ml, 25% agarose | $416.00 | |||
COPG Antibody (H-4) HRP | sc-271362 HRP | 200 µg/ml | $316.00 | |||
COPG Antibody (H-4) FITC | sc-271362 FITC | 200 µg/ml | $330.00 | |||
COPG Antibody (H-4) PE | sc-271362 PE | 200 µg/ml | $343.00 | |||
COPG Antibody (H-4) Alexa Fluor® 488 | sc-271362 AF488 | 200 µg/ml | $357.00 | |||
COPG Antibody (H-4) Alexa Fluor® 546 | sc-271362 AF546 | 200 µg/ml | $357.00 | |||
COPG Antibody (H-4) Alexa Fluor® 594 | sc-271362 AF594 | 200 µg/ml | $357.00 | |||
COPG Antibody (H-4) Alexa Fluor® 647 | sc-271362 AF647 | 200 µg/ml | $357.00 | |||
COPG Antibody (H-4) Alexa Fluor® 680 | sc-271362 AF680 | 200 µg/ml | $357.00 | |||
COPG Antibody (H-4) Alexa Fluor® 790 | sc-271362 AF790 | 200 µg/ml | $357.00 |