COPD Antibody (E-12): sc-515549
- COPD Antibody (E-12) is a mouse monoclonal IgG2a κ COPD antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 254-511 mapping at the C-terminus of COPD of human origin
- COPD Antibody (E-12) is recommended for detection of COPD of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-COPD Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for COPD Antibody (E-12) for WB applications. These reagents are now offered in bundles with COPD Antibody (E-12) (see ordering information below).
COPD Antibody (E-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects COPD protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). COPD (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. COPD protein plays a crucial role in membrane and vesicular trafficking within the early secretory pathway, specifically through the mediation of non-Clathrin coat protein I-coated vesicles. COPI-coated vesicles are essential for retrograde transport from the Golgi apparatus back to the endoplasmic reticulum, as well as for intra-Golgi transport, which is vital for maintaining cellular homeostasis and proper protein sorting. The cytosolic precursor of the COPI coat, known as the heptameric coatomer complex, consists of two subcomplexes: the first includes COPB, COPG, COPD, and COPZ subunits (also referred to as β-, γ-, δ-, and ζ-COP, respectively), which share distant homology with AP Clathrin adaptor subunits, while the second subcomplex comprises COPA, β′-COP, and COPE subunits (also known as α-COP, COPP, and ε-COP, respectively). This intricate structure and function of COPD protein underscore its importance in cellular transport mechanisms, making anti-COPD antibody (E-12) an invaluable tool for researchers studying these processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
COPD Antibody (E-12) References:
- Identification and characterization of novel isoforms of COP I subunits. | Futatsumori, M., et al. 2000. J Biochem. 128: 793-801. PMID: 11056392
- The alpha- and beta'-COP WD40 domains mediate cargo-selective interactions with distinct di-lysine motifs. | Eugster, A., et al. 2004. Mol Biol Cell. 15: 1011-23. PMID: 14699056
- Airway Inflammation Biomarker for Precise Management of Neutrophil-Predominant COPD. | Liang, X., et al. 2020. Methods Mol Biol. 2204: 181-191. PMID: 32710325
- Preclinical studies and the function of IL-17 cytokines in COPD. | Ritzmann, F. and Beisswenger, C. 2021. Ann Anat. 237: 151729. PMID: 33798693
- ACO (Asthma-COPD Overlap) Is Independent from COPD, a Case in Favor: A Systematic Review. | Fujino, N. and Sugiura, H. 2021. Diagnostics (Basel). 11: PMID: 34064650
- Parental COPD as a Risk Factor for the Development of COPD and Disease Severity in Offspring: A Systematic Scoping Review. | Sikjær, MG., et al. 2022. Int J Chron Obstruct Pulmon Dis. 17: 1323-1338. PMID: 35706707
- In vitro assembly and disassembly of coatomer. | Lowe, M. and Kreis, TE. 1995. J Biol Chem. 270: 31364-71. PMID: 8537409
- Delta- and zeta-COP, two coatomer subunits homologous to clathrin-associated proteins, are involved in ER retrieval. | Cosson, P., et al. 1996. EMBO J. 15: 1792-8. PMID: 8617224
- The coatomer protein delta-COP, encoded by the archain gene, is conserved across diverse eukaryotes. | Tunnacliffe, A., et al. 1996. Mamm Genome. 7: 784-6. PMID: 8854871
- Architecture of coatomer: molecular characterization of delta-COP and protein interactions within the complex. | Faulstich, D., et al. 1996. J Cell Biol. 135: 53-61. PMID: 8858162
- Specific interaction of Golgi coatomer protein alpha-COP with phosphatidylinositol 3,4,5-trisphosphate. | Chaudhary, A., et al. 1998. J Biol Chem. 273: 8344-50. PMID: 9525943
- A single binding site for dilysine retrieval motifs and p23 within the gamma subunit of coatomer. | Harter, C. and Wieland, FT. 1998. Proc Natl Acad Sci U S A. 95: 11649-54. PMID: 9751720
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COPD Antibody (E-12) | sc-515549 | 200 µg/ml | $316.00 | |||
COPD Antibody (E-12): m-IgG Fc BP-HRP Bundle | sc-531409 | 200 µg Ab; 10 µg BP | $354.00 | |||
COPD Antibody (E-12): m-IgGκ BP-HRP Bundle | sc-525093 | 200 µg Ab, 40 µg BP | $354.00 | |||
COPD Antibody (E-12) AC | sc-515549 AC | 500 µg/ml, 25% agarose | $416.00 | |||
COPD Antibody (E-12) HRP | sc-515549 HRP | 200 µg/ml | $316.00 | |||
COPD Antibody (E-12) FITC | sc-515549 FITC | 200 µg/ml | $330.00 | |||
COPD Antibody (E-12) PE | sc-515549 PE | 200 µg/ml | $343.00 | |||
COPD Antibody (E-12) Alexa Fluor® 488 | sc-515549 AF488 | 200 µg/ml | $357.00 | |||
COPD Antibody (E-12) Alexa Fluor® 546 | sc-515549 AF546 | 200 µg/ml | $357.00 | |||
COPD Antibody (E-12) Alexa Fluor® 594 | sc-515549 AF594 | 200 µg/ml | $357.00 | |||
COPD Antibody (E-12) Alexa Fluor® 647 | sc-515549 AF647 | 200 µg/ml | $357.00 | |||
COPD Antibody (E-12) Alexa Fluor® 680 | sc-515549 AF680 | 200 µg/ml | $357.00 | |||
COPD Antibody (E-12) Alexa Fluor® 790 | sc-515549 AF790 | 200 µg/ml | $357.00 |