Date published: 2025-9-5
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COPA Antibody (H-3) - Western Blotting - Image 360713
COPA 抗体 (H-3): sc-398099. COPA 在 HeLa (A), U-251-MG (B), Jurkat (C), Hep G2 (D), MCF7 (E) 和 A-10 (F) 全细胞裂解液中的表达的 Western 印迹分析.
COPA 抗体 (H-3): sc-398099. COPA 在 HeLa (A), U-251-MG (B), Jurkat (C), Hep G2 (D), MCF7 (E) 和 A-10 (F) 全细胞裂解液中的表达的 Western 印迹分析.

COPA 抗体 (H-3): sc-398099

4.9(10)
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说明书
  • COPA 抗体 H-3 是小鼠单克隆 IgG2b κ，COPA抗体, 在10篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列934-1233位于human物种的COPA的C-terminus
  • COPA 抗体 (H-3) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 COPA
  • 抗COPA抗体（H-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取COPA (H-3): sc-398099的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP是COPA Antibody (H-3) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与COPA Antibody (H-3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外COP抗体，包括COPA, COPB, COPD, COPE, COPG和COPZ1

COPA抗体（H-3）是一种IgG2b κ型小鼠单克隆COPA抗体（也称为COPA抗体），能够通过WB、IP、IF和ELISA方法检测小鼠、大鼠和人类来源的COPA蛋白。COPA抗体（H-3）既可以是非偶联的抗COPA抗体形式，也可以是多种偶联形式的抗COPA抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。COPA（α-衣被蛋白）经过加工后产生Xenin。Xenin通过刺激外分泌胰腺分泌来影响小肠和大肠的蠕动，并抑制五肽胃泌素刺激的胃酸分泌。在肠道中，Xenin与神经降压素受体相互作用。非网格蛋白COP（衣被蛋白）I包被囊泡介导早期分泌途径中的膜和囊泡转运。COPI包被囊泡介导从高尔基体向内质网和高尔基体内转运的逆行运输。COPI衣被的胞质前体，即七聚体衣被蛋白复合物，由两个亚复合物组成。第一个亚复合物由COPB、COPG、COPD和COPZ亚基（也称为β-、γ-、δ-和ζ-COP）组成，它们与AP网格蛋白适配体亚基具有较远的同源性。第二个亚复合物由COPA、b'-COP和COPE亚基（也称为α-COP、COPP和ε-COP）组成。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

COPA 抗体 (H-3) 参考文献:

  1. Dsl1p是酵母高尔基-内质网检索系统的一个重要组成部分，它利用相同的序列基序与COPI囊泡外壳的不同亚基相互作用。  |  Andag, U. and Schmitt, HD. 2003. J Biol Chem. 278: 51722-34. PMID: 14504276
  2. α-和β'-COP WD40结构域与不同的二赖氨酸基团介导货物选择性相互作用。  |  Eugster, A., et al. 2004. Mol Biol Cell. 15: 1011-23. PMID: 14699056
  3. 衣壳蛋白复合体 160-kD alpha 亚基人类同源物的分子和细胞研究。  |  Quek, HH. and Chow, VT. 1997. DNA Cell Biol. 16: 275-80. PMID: 9115636
  4. α衣壳蛋白 COPA（HEP-COP）：cDNA 中存在 Alu 重复，氨基末端与 xenin 相同。  |  Chow, VT. and Quek, HH. 1997. Ann Hum Genet. 61: 369-73. PMID: 9365789
  5. 高尔基体衣壳蛋白 alpha-COP 与 3,4,5-三磷酸肌醇的特异性相互作用。  |  Chaudhary, A., et al. 1998. J Biol Chem. 273: 8344-50. PMID: 9525943
  6. 谢宁--回顾  |  Feurle, GE. 1998. Peptides. 19: 609-15. PMID: 9533652
  7. Alpha-COP 可以在体外分辨不同的功能性二赖氨酸信号，并调节进入逆向运输的途径。  |  Schröder-Köhne, S., et al. 1998. J Cell Sci. 111 (Pt 23): 3459-70. PMID: 9811561

订购信息

产品名称产品编号规格价格数量收藏夹

COPA 抗体 (H-3)

sc-398099
200 µg/ml
$316.00

COPA (H-3): m-IgG2b BP-HRP 套装

sc-548785
200 µg Ab; 10 µg BP
$354.00

COPA 抗体 (H-3) AC

sc-398099 AC
500 µg/ml, 25% agarose
$416.00

COPA 抗体 (H-3) HRP

sc-398099 HRP
200 µg/ml
$316.00

COPA 抗体 (H-3) FITC

sc-398099 FITC
200 µg/ml
$330.00

COPA 抗体 (H-3) PE

sc-398099 PE
200 µg/ml
$343.00

COPA 抗体 (H-3) Alexa Fluor® 488

sc-398099 AF488
200 µg/ml
$357.00

COPA 抗体 (H-3) Alexa Fluor® 546

sc-398099 AF546
200 µg/ml
$357.00

COPA 抗体 (H-3) Alexa Fluor® 594

sc-398099 AF594
200 µg/ml
$357.00

COPA 抗体 (H-3) Alexa Fluor® 647

sc-398099 AF647
200 µg/ml
$357.00

COPA 抗体 (H-3) Alexa Fluor® 680

sc-398099 AF680
200 µg/ml
$357.00

COPA 抗体 (H-3) Alexa Fluor® 790

sc-398099 AF790
200 µg/ml
$357.00

How much protein do you recommend loading on the gel for Western blot detection using COPA (H-3): sc-398099 monoclonal antibody?

Asked by: jenniferc
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Santa Cruz TS
Date published: 2017-02-02
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Rated 5 out of 5 by from To be recommendedI bought this Antibody-COPA for IP-WB. It works properly.
Date published: 2022-08-17
Rated 5 out of 5 by from perfect by western blotting (MCF7 cells)I used this antibody for western blotting on MCF7 cell extracts (dilution 1:1000, TBST-5%milk). It produces a clear band at the described size, using normal ECL reagent.
Date published: 2020-02-14
Rated 4 out of 5 by from Great for IF and WB but not IPThis CopA antibody stained Golgi and vesicular pre-Golgi compartments in both HeLa and COS7 cells very nicely. It works with both MeOH and PFA followed by detergent extraction. I also tried doing IP, but didn't work in my hands.
Date published: 2018-11-30
Rated 5 out of 5 by from Works wellI got this as a free trial sample and used it with primary cortical neuron samples. The bands were robust with only 10ug loaded/lane and there were no secondary bands.
Date published: 2018-02-26
Rated 5 out of 5 by from Funziona benissimo!Ho testato questo Ab in Immunofluorescenza su fibroblasti immortalizzati ed ho ottenuto immagini molto belle, con un pattern di marcatura specifico e pulito. Lo consiglio!
Date published: 2017-11-15
Rated 5 out of 5 by from Excellent anticorps !J'ai utilisé cet anticorps dans des cellules HEK293T : anticorps spécifique et sensible.
Date published: 2017-10-17
Rated 5 out of 5 by from Excellent for WBit works excellent for WB in our lab, will keep usinig it
Date published: 2017-09-18
Rated 5 out of 5 by from Clean and clear bands detectedClean and distinct bands detected by Western blot in multiple cell lines. Highly recommended
Date published: 2017-01-06
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COPA 抗体 (H-3) is rated 4.9 out of 5 by 10.
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