COP1 Antibody (B-12): sc-166799
- COP1 Antibody (B-12) is a mouse monoclonal IgG1 κ COP1 antibody, cited in 7 publications, provided at 200 µg/ml
- raised against amino acids 432-731 mapping at the C-terminus of COP1 of human origin
- COP1 Antibody (B-12) is recommended for detection of COP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-COP1 Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-166799 X, 200 µg/0.1 ml)
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for COP1 Antibody (B-12) for WB and IHC(P) applications. These reagents are now offered in bundles with COP1 Antibody (B-12) (see ordering information below).
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SEE ALSO...
COP1 (B-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects COP1 protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP) and enzyme-linked immunosorbent assay (ELISA). COP1 (B-12) antibody is available as both non-conjugated form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. COP1 (Constitutive photomorphogenesis protein 1), also designated RFWD2 (ring finger and WD repeat domain 2) or RNF200 (ring finger protein 200), is an E3 ubiquitin ligase protein that mediates ubiquitination and degradation of target proteins such as c-Jun and p53. COP1 functions as a component of the DCX DET1-COP1 ubiquitin ligase complex which consists of RBX1, DET1, DDB1, CUL4A and COP1. COP1 localizes to the cytoplasm and nucleus, with primary expression in testis, placenta, heart and skeletal muscle. COP1 acts as a potent inhibitor of p53-dependent transcription and apoptosis but, when phosphorylated by Atm (ataxia telangiectasia mutated) in response to DNA damage, the COP1-p53 complex is disrupted and p53 is allowed to exert its pro-apoptotic properties. COP1 shows overexpression in ovarian and breast cancers, suggesting a role in tumorigenesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
COP1 Antibody (B-12) References:
- The ubiquitin ligase COP1 is a critical negative regulator of p53. | Dornan, D., et al. 2004. Nature. 429: 86-92. PMID: 15103385
- ARF1 regulates Nef-induced CD4 degradation. | Fauré, J., et al. 2004. Curr Biol. 14: 1056-64. PMID: 15202998
- Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation. | Feng, S., et al. 2004. Plant Cell. 16: 1870-82. PMID: 15208391
- COP and clathrin-coated vesicle budding: different pathways, common approaches. | McMahon, HT. and Mills, IG. 2004. Curr Opin Cell Biol. 16: 379-91. PMID: 15261670
- COP1, the negative regulator of p53, is overexpressed in breast and ovarian adenocarcinomas. | Dornan, D., et al. 2004. Cancer Res. 64: 7226-30. PMID: 15492238
- Major vault protein, in concert with constitutively photomorphogenic 1, negatively regulates c-Jun-mediated activator protein 1 transcription in mammalian cells. | Yi, C., et al. 2005. Cancer Res. 65: 5835-40. PMID: 15994960
- TRB3 links the E3 ubiquitin ligase COP1 to lipid metabolism. | Qi, L., et al. 2006. Science. 312: 1763-6. PMID: 16794074
- ATM engages autodegradation of the E3 ubiquitin ligase COP1 after DNA damage. | Dornan, D., et al. 2006. Science. 313: 1122-6. PMID: 16931761
- Role of the COP1 protein in cancer development and therapy. | Song, Y., et al. 2020. Semin Cancer Biol. 67: 43-52. PMID: 32027978
- Ubiquitin Ligase COP1 Suppresses Neuroinflammation by Degrading c/EBPβ in Microglia. | Ndoja, A., et al. 2020. Cell. 182: 1156-1169.e12. PMID: 32795415
- A COP1-GATA2 axis suppresses AR signaling and prostate cancer. | Shen, T., et al. 2022. Proc Natl Acad Sci U S A. 119: e2205350119. PMID: 36251994
- IL-37d enhances COP1-mediated C/EBPβ degradation to suppress spontaneous neutrophil migration and tumor progression. | Guo, Y., et al. 2024. Cell Rep. 43: 113787. PMID: 38363681
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COP1 Antibody (B-12) | sc-166799 | 200 µg/ml | $316.00 | |||
COP1 Antibody (B-12): m-IgGκ BP-HRP Bundle | sc-521751 | 200 µg Ab, 40 µg BP | $354.00 | |||
COP1 Antibody (B-12): m-IgG1 BP-HRP Bundle | sc-533505 | 200 µg Ab; 20 µg BP | $354.00 | |||
COP1 Antibody (B-12) X | sc-166799 X | 200 µg/0.1 ml | $316.00 | |||
COP1 Antibody (B-12) AC | sc-166799 AC | 500 µg/ml, 25% agarose | $416.00 | |||
COP1 Antibody (B-12) HRP | sc-166799 HRP | 200 µg/ml | $316.00 | |||
COP1 Antibody (B-12) FITC | sc-166799 FITC | 200 µg/ml | $330.00 | |||
COP1 Antibody (B-12) PE | sc-166799 PE | 200 µg/ml | $343.00 | |||
COP1 Antibody (B-12) Alexa Fluor® 488 | sc-166799 AF488 | 200 µg/ml | $357.00 | |||
COP1 Antibody (B-12) Alexa Fluor® 546 | sc-166799 AF546 | 200 µg/ml | $357.00 | |||
COP1 Antibody (B-12) Alexa Fluor® 594 | sc-166799 AF594 | 200 µg/ml | $357.00 | |||
COP1 Antibody (B-12) Alexa Fluor® 647 | sc-166799 AF647 | 200 µg/ml | $357.00 | |||
COP1 Antibody (B-12) Alexa Fluor® 680 | sc-166799 AF680 | 200 µg/ml | $357.00 | |||
COP1 Antibody (B-12) Alexa Fluor® 790 | sc-166799 AF790 | 200 µg/ml | $357.00 |