connexin 50 Antibody (B-11): sc-373801
- connexin 50 Antibody (B-11) is a mouse monoclonal IgG1 κ connexin 50 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 228-292 mapping within a cytoplasmic domain of connexin 50 of human origin
- connexin 50 Antibody (B-11) is recommended for detection of connexin 50 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-connexin 50 Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for connexin 50 Antibody (B-11) for WB applications. These reagents are now offered in bundles with connexin 50 Antibody (B-11) (see ordering information below).
QUICK LINKS
SEE ALSO...
connexin 50 Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects connexin 50 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-connexin 50 antibody (B-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Connexin 50, also known as gap junction protein alpha 8, plays a crucial role in forming gap junctions, which are essential for intercellular communication. Gap junctions allow the passage of ions and small molecules between adjacent cells, facilitating coordinated cellular responses and maintaining tissue homeostasis. Connexin 50 is particularly important in the lens of the eye, where connexin 50 contributes to the transparency and refractive properties necessary for proper vision. Mutations or deletions in the gene encoding connexin 50 can lead to cataracts, highlighting connexin 50′s significance in ocular health. The unique structure of connexin proteins, characterized by four transmembrane alpha-helical domains and specific extracellular loops, allows for the formation of functional channels that vary in permeability and sensitivity, underscoring connexin 50′s importance in both normal physiology and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
connexin 50 Antibody (B-11) References:
- Emerging issues of connexin channels: biophysics fills the gap. | Harris, AL. 2001. Q Rev Biophys. 34: 325-472. PMID: 11838236
- Dominant cataracts result from incongruous mixing of wild-type lens connexins. | Martinez-Wittinghan, FJ., et al. 2003. J Cell Biol. 161: 969-78. PMID: 12782682
- Human cardiac gap junctions: isolation, ultrastructure, and protein composition. | Manjunath, CK., et al. 1987. J Mol Cell Cardiol. 19: 131-4. PMID: 3573042
- Decreased connexin expression and intercellular communication in human bladder cancer cells. | Grossman, HB., et al. 1994. Cancer Res. 54: 3062-5. PMID: 8187096
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
connexin 50 Antibody (B-11) | sc-373801 | 200 µg/ml | $316.00 | |||
connexin 50 Antibody (B-11): m-IgG Fc BP-HRP Bundle | sc-527414 | 200 µg Ab; 10 µg BP | $354.00 | |||
connexin 50 Antibody (B-11): m-IgG1 BP-HRP Bundle | sc-532787 | 200 µg Ab; 20 µg BP | $354.00 | |||
connexin 50 Antibody (B-11) AC | sc-373801 AC | 500 µg/ml, 25% agarose | $416.00 | |||
connexin 50 Antibody (B-11) HRP | sc-373801 HRP | 200 µg/ml | $316.00 | |||
connexin 50 Antibody (B-11) FITC | sc-373801 FITC | 200 µg/ml | $330.00 | |||
connexin 50 Antibody (B-11) PE | sc-373801 PE | 200 µg/ml | $343.00 | |||
connexin 50 Antibody (B-11) Alexa Fluor® 488 | sc-373801 AF488 | 200 µg/ml | $357.00 | |||
connexin 50 Antibody (B-11) Alexa Fluor® 546 | sc-373801 AF546 | 200 µg/ml | $357.00 | |||
connexin 50 Antibody (B-11) Alexa Fluor® 594 | sc-373801 AF594 | 200 µg/ml | $357.00 | |||
connexin 50 Antibody (B-11) Alexa Fluor® 647 | sc-373801 AF647 | 200 µg/ml | $357.00 | |||
connexin 50 Antibody (B-11) Alexa Fluor® 680 | sc-373801 AF680 | 200 µg/ml | $357.00 | |||
connexin 50 Antibody (B-11) Alexa Fluor® 790 | sc-373801 AF790 | 200 µg/ml | $357.00 |