connexin 43 Antibody (D-7): sc-13558

Connexin 43 Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects connexin 43 in mouse, rat, human, and canine samples through western blotting (WB) and immunoprecipitation (IP). Anti-connexin 43 antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Connexin 43, also known as GJA1, is a critical gap junction protein that forms hexameric structures called connexons, which cluster to create gap junctions facilitating intercellular communication. This communication is vital for maintaining tissue homeostasis and coordinating cellular responses, particularly in cardiac and neuronal tissues. Notably, connexin 43 is subject to various post-translational modifications, including phosphorylation, which can significantly influence function. During mitosis, connexin 43 undergoes specific phosphorylation that correlates with a temporary loss of gap junction intercellular communication, highlighting dynamic roles in cellular signaling and regulation of cell proliferation. In pathological conditions, such as cancer, altered expression and function of connexin 43 can lead to impaired gap junction communication, contributing to tumor progression and metastasis. Anti-connexin 43 antibody (D-7) effectively detects this crucial protein, making connexin 43 monoclonal antibody (D-7) an essential tool for researchers studying cell communication, cancer biology, and physiological roles of gap junctions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

connexin 43 Antibody (D-7) References:

1. Emerging issues of connexin channels: biophysics fills the gap.  |  Harris, AL. 2001. Q Rev Biophys. 34: 325-472. PMID: 11838236
2. The cardiac gap junction: a potential therapeutic target in the treatment of heart disease.  |  Liu, F. and Gutstein, DE. 2002. Mt Sinai J Med. 69: 421-4. PMID: 12429963
3. Change in permeant size selectivity by phosphorylation of connexin 43 gap-junctional hemichannels by PKC.  |  Bao, X., et al. 2007. Proc Natl Acad Sci U S A. 104: 4919-24. PMID: 17360407
4. LM and EM immunolocalization of the gap junctional protein connexin 43 in rat brain.  |  Yamamoto, T., et al. 1990. Brain Res. 508: 313-9. PMID: 2155040
5. Aberrant expression of gap junction gene in primary human hepatocellular carcinomas: increased expression of cardiac-type gap junction gene connexin 43.  |  Oyamada, M., et al. 1990. Mol Carcinog. 3: 273-8. PMID: 2173931
6. Retinoid-enhanced gap junctional communication is achieved by increased levels of connexin 43 mRNA and protein.  |  Rogers, M., et al. 1990. Mol Carcinog. 3: 335-43. PMID: 2177604
7. Differential expression of three gap junction proteins in developing and mature brain tissues.  |  Dermietzel, R., et al. 1989. Proc Natl Acad Sci U S A. 86: 10148-52. PMID: 2557621
8. Connexin 43 and Mitochondria in Cardiovascular Health and Disease.  |  Boengler, K. and Schulz, R. 2017. Adv Exp Med Biol. 982: 227-246. PMID: 28551790
9. Function of Connexin-43 in Macrophages.  |  Rodjakovic, D., et al. 2021. Int J Mol Sci. 22: PMID: 33573367
10. Human cardiac gap junctions: isolation, ultrastructure, and protein composition.  |  Manjunath, CK., et al. 1987. J Mol Cell Cardiol. 19: 131-4. PMID: 3573042
11. FGF21 protects against doxorubicin-induced cardiotoxicity by inhibiting connexin 43 ubiquitination.  |  Huang, Y., et al. 2023. Free Radic Biol Med. 208: 748-758. PMID: 37774805
12. Decreased connexin expression and intercellular communication in human bladder cancer cells.  |  Grossman, HB., et al. 1994. Cancer Res. 54: 3062-5. PMID: 8187096

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-connexin 43 Antibody (D-7) has 35 citations in a variety of scientific publications.

Primary antibodies like Anti-connexin 43 Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

connexin 43 Antibody (D-7) Specifications

Alternate Product Name: GJA1

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 241-254

Epitope Target: connexin 43

Epitope Species: rat

Product Reactivity/Detection: connexin 43

Species Reactivity/Detection: mouse, rat, human, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 35 citations in various scientific publications

Available Conjugates/Forms for connexin 43 Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin