Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
connexin 26 CRISPR/Cas9 KO Plasmid (m): sc-420565
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- connexin 26 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the connexin 26 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: connexin 26 Antibody (P1F5): sc-517456
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
connexin 26 CRISPR/Cas9 KO Plasmid (m) | sc-420565 | 20 µg | $397.00 |
Overview
Gjb2 encodes connexin 26 (Cx26), a gap junction protein that assembles into hexameric connexons to form intercellular channels enabling direct exchange of ions and small metabolites. By coordinating electrical and metabolic coupling, Cx26 supports tissue homeostasis, epithelial barrier function, and synchronization of cellular responses to stress and differentiation cues. Connexin 26 participates in processes linked to calcium signaling, redox balance, and contact-mediated regulation of proliferation and apoptosis. Disrupted Gjb2 function is strongly associated with syndromic and nonsyndromic hearing impairment and is also implicated in disorders of epidermal differentiation where altered gap-junctional communication contributes to pathology.
connexin 26 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Gjb2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Gjb2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Gjb2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish connexin 26 protein expression.
This CRISPR knockout system enables efficient generation of Gjb2-deficient cell models for investigation of connexin 26 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Gjb2 exon(s) critical for connexin 26 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Gjb2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by connexin 26 CRISPR/Cas9 KO Plasmid (m) and connexin 26 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Gjb2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by connexin 26 HDR Plasmid (m) and connexin 26 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Gjb2 homology arms to support homology-directed repair at defined Gjb2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.