Date published: 2025-11-21
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COL1A Antibody (COL-1) - Immunofluorescence - Image 145258
COL1A Antibody (COL-1): sc-59772. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.
COL1A Antibody (COL-1) - Immunofluorescence - Image 145258
COL1A Antibody (COL-1): sc-59772. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.
COL1A Antibody (COL-1): sc-59772. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.

COL1A Antibody (COL-1): sc-59772

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Datasheets
  • COL1A Antibody (COL-1) is a mouse monoclonal IgG1 COL1A antibody, cited in 222 publications, provided at 100 µl ascites
  • raised against full length native Collagen Type I of bovine origin
  • recommended for detection of native Collagen Type I of mouse, rat and human origin by WB (non-reducing), IP, IF, IHC (frozen) and ELISA; also reactive with additional species, including and bovine, porcine, feline and canine; may cross-react with connective tissue fibers in acetone-fixed or unfixed frozen sections; non cross-reactive with Collagen Types II, III, IV, V, VI, VII, IX, X and XI
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for COL1A Antibody (COL-1). This work is in progress.

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SEE ALSO...

Additional Collagen Antibodies including COL10A1, COL12A1, COL14A1, COL17A1, COL18A1, COL1A1, COL1A2, COL23A1, COL2A1 and COL3A1

COL1A Antibody (COL-1) is a mouse monoclonal IgG1 antibody that detects COL1A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-COL1A antibody (COL-1) is available as the non-conjugated format. COL1A protein, also known as collagen type I alpha 1 chain, is a crucial component of the extracellular matrix and plays a vital role in providing structural support to various tissues, including bone, cartilage, tendon, and skin. A unique triple helix structure is essential for COL1A function, allowing for stable fibril formation that contributes to connective tissue tensile strength. This structural integrity maintains tissue mechanical properties under stress, and mutations or deficiencies in COL1A can lead to conditions like osteogenesis imperfecta, characterized by fragile bones. The extensive collagen protein family, including COL1A, is fundamental to tissue architecture and cellular interactions, making COL1A research significant for understanding various developmental and pathological processes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

COL1A Antibody (COL-1) References:

  1. Collagen and bone strength.  |  Boskey, AL., et al. 1999. J Bone Miner Res. 14: 330-5. PMID: 10027897
  2. Review article: platelet-collagen interactions: membrane receptors and intracellular signalling pathways.  |  Alberio, L. and Dale, GL. 1999. Eur J Clin Invest. 29: 1066-76. PMID: 10583456
  3. Association of the collagen type 1 (COL1A 1) Sp1 binding site polymorphism to femoral neck bone mineral density and wrist fracture in 1044 elderly Swedish women.  |  Gerdhem, P., et al. 2004. Calcif Tissue Int. 74: 264-9. PMID: 14595528
  4. Transforming-growth-factor-beta activation elements in the distal promoter regions of the rat alpha 1 type I collagen gene.  |  Ritzenthaler, JD., et al. 1991. Biochem J. 280 (Pt 1): 157-62. PMID: 1741743
  5. The prognostic potential of alpha-1 type I collagen expression in papillary thyroid cancer.  |  Huang, C., et al. 2019. Biochem Biophys Res Commun. 515: 125-132. PMID: 31128912
  6. Prognostic Prediction, Immune Microenvironment, and Drug Resistance Value of Collagen Type I Alpha 1 Chain: From Gastrointestinal Cancers to Pan-Cancer Analysis.  |  Liu, Y., et al. 2021. Front Mol Biosci. 8: 692120. PMID: 34395525
  7. Col1A-2 Mutation in Osteogenesis Imperfecta Mice Contributes to Long Bone Fragility by Modifying Cell-Matrix Organization.  |  André, G., et al. 2023. Int J Mol Sci. 24: PMID: 38069332
  8. Cell adhesion to collagenous matrices.  |  McCarthy, JB., et al. 1996. Biopolymers. 40: 371-81. PMID: 8765608
  9. Nasal tolerance to dominant and subdominant epitopes of collagen type II and protection against collagen-induced arthritis.  |  Staines, NA., et al. 1997. Biochem Soc Trans. 25: 661-4. PMID: 9191176
  10. Versatile collagens in invertebrates.  |  Engel, J. 1997. Science. 277: 1785-6. PMID: 9324767
  11. Collagen-induced arthritis, an animal model of autoimmunity.  |  Myers, LK., et al. 1997. Life Sci. 61: 1861-78. PMID: 9364191
  12. The cartilage collagens: a review of their structure, organization, and role in the pathogenesis of experimental arthritis in animals and in human rheumatic disease.  |  Cremer, MA., et al. 1998. J Mol Med (Berl). 76: 275-88. PMID: 9535561

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

COL1A Antibody (COL-1)

sc-59772
100 µl ascites
$316.00

Does this antibody(59772) detect collagen1 or col1a1? What is the difference between this antibody and 293182?

Asked by: gene
Thank you for your question. sc-59772 is recommended for detection of native Collagen Type I of mouse, rat and human origin by WB (non-reducing), IP, IF, IHC (frozen) and ELISA. If you have any other question, please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Sen Li
Date published: 2025-09-04

Hi, can you suggest a protocol of western blot for detecting this native collagen from porcine samples?

Asked by: danK
Thank you for your question. You can find our recommended western blot protocol under this link: https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: Tech Support Europe
Date published: 2021-02-03

Hello, is there an SDS document for this product? Our Health & Safety department requires one for all products we use.

Asked by: J Thorpe
Thank you for your question. We do not provide SDS documents for our antibody reagents. SDSs are only provided for chemicals. Please reach out to your local distributor, they will be able to further assist you.
Answered by: Tech Service
Date published: 2020-01-28

What is the dilution used for immunohistochemistry (P)? Thank you

Asked by: marcelnani
Thank you for your question. We recommend a starting dilution of 1:50 for staining paraffin embedded sections with this antibody.
Answered by: Tech Service
Date published: 2021-06-04

For WB, does this antibody only recognizes native proteins? No SDS and reducing reagents in loading buffer?

Asked by: qinggoushi
Thank you for your question. This antibody, COL1A, sc-59772, will only recognize the native form, so yes, do not add SDS or reducing reagents to the loading buffer.
Answered by: Tech Service
Date published: 2019-11-13

If there any recommandation of secondary antibody for IF?

Asked by: lucky7
Thank you for your question. COL1A Antibody (COL-1) is a mouse monoclonal IgG1. We recommend using as a secondary antibody for IF, our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to a fluorescent probe such as fluorescein (FITC), m-IgGκ BP-FITC: sc-516140.
Answered by: Technical Support
Date published: 2019-04-13

Will this antibody stain for secreted fibrillar collagen-1? I'm unsure whether full-length native collagen type-1 means its specific for assembled collagen-1 fibrils. Will it also stain for pro-collagen chains? or only assembled fibrils? Thanks!

Asked by: Benn
Thank you for your question. This COL1A antibody recognises the native (helical) form of collagen type I.
Answered by: Tech Service
Date published: 2019-03-26

Hi, Can you supply conjugated version (either Alexa or PE) of this COL1A Antibody (COL-1): sc-59772? Thanks

Asked by: Harinath Sale
Thank you for your question. No, this antibody is provided in an ascites format and therefore is not available as a conjugated antibody. Please contact our Technical Service for recommended alternatives.
Answered by: Technical Support
Date published: 2018-12-13
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Rated 5 out of 5 by from 出来结果不错我是上学时候用的抗体，很不错，现在工作了继续实验，又定了点抗体，价格可以，实验也满意，非常不错，赞。。
Date published: 2024-12-25
Rated 1 out of 5 by from no good for MEFI bought few weeks ago. I did western blot as soon as I got it. I used this antibody by dilution 1:500. There was no specific collagen band. I only tried MEF lysate. I'm not sure this antibody works for mouse collagen. If anybody knows antibodies for mouse collagen, please give me any information. Thanks,
Date published: 2018-05-11
Rated 5 out of 5 by from goodI tried this antibody in WB and got a very clear result
Date published: 2017-09-22
Rated 5 out of 5 by from for WBThis COL1A antibody by dilution 1:1000, WB results are good.
Date published: 2017-05-11
Rated 5 out of 5 by from specifically stains collagen IThis antibody specifically stains collagen I, which is an extra cellular matrix protein. As a result, the cell boundary of cardiomyocytes was clearly stained.
Date published: 2017-01-20
Rated 3 out of 5 by from Col 1a antibodyI bought few month ago and did western blot. I got some time good signal but these day I don't detect the signal. I have no idea why signal is suddenly disappear.
Date published: 2016-12-27
Rated 5 out of 5 by from Very good native detectionBest for native detection, and is specific for only COL1A
Date published: 2016-12-21
Rated 4 out of 5 by from No issues in our WesternNo issues in our Western. Good signal.
Date published: 2015-08-20
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COL1A Antibody (COL-1) is rated 4.5 out of 5 by 13.
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