Cofilin 2 Antibody (D-5): sc-166958
- Cofilin 2 Antibody (D-5) is a mouse monoclonal IgG1 κ Cofilin 2 antibody, cited in 4 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 133-151 near the C-terminus of Cofilin 2 of human origin
- Cofilin 2 Antibody (D-5) is recommended for detection of Cofilin 2, muscle isoform of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
- Anti-Cofilin 2 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cofilin 2 Antibody (D-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Cofilin 2 Antibody (D-5) (see ordering information below).
Cofilin 2 Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cofilin 2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Cofilin 2 antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cofilin 2, also referred to as CFL2 or Cofilin, muscle isoform, is a 166 amino acid protein that plays a crucial role in regulating actin dynamics within the cytoplasm and cytoskeleton, as well as in the nuclear matrix, where Cofilin 2 contains one actin-depolymerizing factor homology (ADF-H) domain. This protein exists in two alternatively spliced isoforms: CFL2a, predominantly expressed in heart and skeletal muscle, and CFL2b, which is ubiquitously expressed. Cofilin 2 binds to both filamentous (F) and globular (G) actin, facilitating pH-dependent actin polymerization and depolymerization, which is vital for various cellular processes, including motility, division, and shape maintenance. Notably, Cofilin 2 undergoes post-translational phosphorylation at Serine 24, a modification that can inhibit nuclear localization, thereby influencing functional dynamics. Mutations in the gene encoding Cofilin 2 are associated with nemaline myopathy type 7 (NEM7), a congenital myopathy characterized by the presence of abnormal thread- or rod-like structures in muscle fibers, highlighting the importance of Cofilin 2 in muscle integrity and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cofilin 2 Antibody (D-5) References:
- Putting a new twist on actin: ADF/cofilins modulate actin dynamics. | Bamburg, JR., et al. 1999. Trends Cell Biol. 9: 364-70. PMID: 10461190
- Characterization of human muscle type cofilin (CFL2) in normal and regenerating muscle. | Thirion, C., et al. 2001. Eur J Biochem. 268: 3473-82. PMID: 11422377
- The three mouse actin-depolymerizing factor/cofilins evolved to fulfill cell-type-specific requirements for actin dynamics. | Vartiainen, MK., et al. 2002. Mol Biol Cell. 13: 183-94. PMID: 11809832
- The ADF/cofilin family: actin-remodeling proteins. | Maciver, SK. and Hussey, PJ. 2002. Genome Biol. 3: reviews3007. PMID: 12049672
- Control of growth cone motility and morphology by LIM kinase and Slingshot via phosphorylation and dephosphorylation of cofilin. | Endo, M., et al. 2003. J Neurosci. 23: 2527-37. PMID: 12684437
- Nemaline myopathy with minicores caused by mutation of the CFL2 gene encoding the skeletal muscle actin-binding protein, cofilin-2. | Agrawal, PB., et al. 2007. Am J Hum Genet. 80: 162-7. PMID: 17160903
- Mapping of human non-muscle type cofilin (CFL1) to chromosome 11q13 and muscle-type cofilin (CFL2) to chromosome 14. | Gillett, GT., et al. 1996. Ann Hum Genet. 60: 201-11. PMID: 8800436
- Dephosphorylation of serine 3 regulates nuclear translocation of cofilin. | Nebl, G., et al. 1996. J Biol Chem. 271: 26276-80. PMID: 8824278
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cofilin 2 Antibody (D-5) | sc-166958 | 200 µg/ml | $322.00 | |||
Cofilin 2 Antibody (D-5): m-IgG Fc BP-HRP Bundle | sc-529094 | 200 µg Ab; 10 µg BP | $361.00 | |||
Cofilin 2 Antibody (D-5): m-IgGκ BP-HRP Bundle | sc-521810 | 200 µg Ab, 40 µg BP | $361.00 | |||
Cofilin 2 Antibody (D-5): m-IgG1 BP-HRP Bundle | sc-543314 | 200 µg Ab; 20 µg BP | $361.00 | |||
Cofilin 2 Antibody (D-5) AC | sc-166958 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Cofilin 2 Antibody (D-5) HRP | sc-166958 HRP | 200 µg/ml | $322.00 | |||
Cofilin 2 Antibody (D-5) FITC | sc-166958 FITC | 200 µg/ml | $336.00 | |||
Cofilin 2 Antibody (D-5) PE | sc-166958 PE | 200 µg/ml | $349.00 | |||
Cofilin 2 Antibody (D-5) Alexa Fluor® 488 | sc-166958 AF488 | 200 µg/ml | $364.00 | |||
Cofilin 2 Antibody (D-5) Alexa Fluor® 546 | sc-166958 AF546 | 200 µg/ml | $364.00 | |||
Cofilin 2 Antibody (D-5) Alexa Fluor® 594 | sc-166958 AF594 | 200 µg/ml | $364.00 | |||
Cofilin 2 Antibody (D-5) Alexa Fluor® 647 | sc-166958 AF647 | 200 µg/ml | $364.00 | |||
Cofilin 2 Antibody (D-5) Alexa Fluor® 680 | sc-166958 AF680 | 200 µg/ml | $364.00 | |||
Cofilin 2 Antibody (D-5) Alexa Fluor® 790 | sc-166958 AF790 | 200 µg/ml | $364.00 | |||
Cofilin 2 (D-5) Neutralizing Peptide | sc-166958 P | 100 µg/0.5 ml | $69.00 |