COASY Antibody (E-7): sc-393812
- COASY Antibody (E-7) is a mouse monoclonal IgG2a κ COASY antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of COASY of human origin
- COASY Antibody (E-7) is recommended for detection of COASY of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-COASY Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for COASY Antibody (E-7) for WB applications. These reagents are now offered in bundles with COASY Antibody (E-7) (see ordering information below).
QUICK LINKS
SEE ALSO...
COASY Antibody (E-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects COASY protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). COASY (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. COASY, also known as coenzyme A synthase, plays a crucial role in biosynthesis of coenzyme A (CoA), a vital cofactor involved in numerous metabolic pathways, including carbohydrate, amino acid, and fatty acid metabolism. COASY exhibits bifunctional enzymatic activity, specifically catalyzing conversion of 4′-phosphopantetheine to dephospho-CoA and subsequently generating CoA, which serves as predominant carrier of acetyl and acyl groups in cellular processes. COASY is primarily localized to outer mitochondrial membrane, where COASY is induced by phospholipids, highlighting COASY′s importance in energy metabolism and cellular function. Additionally, due to alternative splicing, an isoform known as COASY b is expressed in brain, featuring an extra 29 amino acids at N-terminus, which may influence functional properties and interactions within cellular environment.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
COASY Antibody (E-7) References:
- Complete reconstitution of the human coenzyme A biosynthetic pathway via comparative genomics. | Daugherty, M., et al. 2002. J Biol Chem. 277: 21431-9. PMID: 11923312
- Molecular cloning of CoA Synthase. The missing link in CoA biosynthesis. | Zhyvoloup, A., et al. 2002. J Biol Chem. 277: 22107-10. PMID: 11980892
- Identification and characterization of the gene encoding the human phosphopantetheine adenylyltransferase and dephospho-CoA kinase bifunctional enzyme (CoA synthase). | Aghajanian, S. and Worrall, DM. 2002. Biochem J. 365: 13-8. PMID: 11994049
- Subcellular localization and regulation of coenzyme A synthase. | Zhyvoloup, A., et al. 2003. J Biol Chem. 278: 50316-21. PMID: 14514684
- Specific interaction between S6K1 and CoA synthase: a potential link between the mTOR/S6K pathway, CoA biosynthesis and energy metabolism. | Nemazanyy, I., et al. 2004. FEBS Lett. 578: 357-62. PMID: 15589845
- Identification of a novel CoA synthase isoform, which is primarily expressed in the brain. | Nemazanyy, I., et al. 2006. Biochem Biophys Res Commun. 341: 995-1000. PMID: 16460672
- Haplotype analysis of the HSD17B1 gene and risk of breast cancer: a comprehensive approach to multicenter analyses of prospective cohort studies. | Feigelson, HS., et al. 2006. Cancer Res. 66: 2468-75. PMID: 16489054
- Characterizing variation in sex steroid hormone pathway genes in women of 4 races/ethnicities: the Study of Women's Health Across the Nation (SWAN). | Kardia, SR., et al. 2006. Am J Med. 119: S3-15. PMID: 16949386
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COASY Antibody (E-7) | sc-393812 | 200 µg/ml | $316.00 | |||
COASY Antibody (E-7): m-IgG Fc BP-HRP Bundle | sc-530587 | 200 µg Ab; 10 µg BP | $354.00 | |||
COASY Antibody (E-7): m-IgGκ BP-HRP Bundle | sc-524097 | 200 µg Ab, 40 µg BP | $354.00 | |||
COASY Antibody (E-7): m-IgG2a BP-HRP Bundle | sc-547526 | 200 µg Ab; 10 µg BP | $354.00 | |||
COASY Antibody (E-7) AC | sc-393812 AC | 500 µg/ml, 25% agarose | $416.00 | |||
COASY Antibody (E-7) HRP | sc-393812 HRP | 200 µg/ml | $316.00 | |||
COASY Antibody (E-7) FITC | sc-393812 FITC | 200 µg/ml | $330.00 | |||
COASY Antibody (E-7) PE | sc-393812 PE | 200 µg/ml | $343.00 | |||
COASY Antibody (E-7) Alexa Fluor® 488 | sc-393812 AF488 | 200 µg/ml | $357.00 | |||
COASY Antibody (E-7) Alexa Fluor® 546 | sc-393812 AF546 | 200 µg/ml | $357.00 | |||
COASY Antibody (E-7) Alexa Fluor® 594 | sc-393812 AF594 | 200 µg/ml | $357.00 | |||
COASY Antibody (E-7) Alexa Fluor® 647 | sc-393812 AF647 | 200 µg/ml | $357.00 | |||
COASY Antibody (E-7) Alexa Fluor® 680 | sc-393812 AF680 | 200 µg/ml | $357.00 | |||
COASY Antibody (E-7) Alexa Fluor® 790 | sc-393812 AF790 | 200 µg/ml | $357.00 |