CNTF Antibody (C-12): sc-166273

CNTF Antibody (C-12) is a mouse monoclonal IgG1 antibody that detects ciliary neurotrophic factor (CNTF) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CNTF is a crucial neuropoietic cytokine that plays a significant role in promoting the survival and differentiation of various cell types, including sensory, sympathetic, and motor neurons, which is vital for maintaining nervous system integrity. CNTF belongs to a cytokine family including leukemia inhibitory factor (LIF) and interleukin-6 (IL-6), sharing structural similarities and utilizing common receptor components for signaling. The CNTF receptor complex, composed of CNTFRα, a specific chain for CNTF, and a heterodimer of gp130 chain and LIFRβ chain, mediates CNTF effects. This complex enhances DNA synthesis by activating transcription factors Stat1 and Stat3, which are critical for cellular responses. CNTF has been implicated in amyotrophic lateral sclerosis (ALS) pathogenesis, highlighting CNTF′s importance in neurodegenerative diseases. Research indicates that while mice lacking CNTF exhibit certain deficiencies, those with a homozygous null mutation in CNTFRα experience perinatal mortality and severe motor neuron deficits, suggesting CNTFRα may have additional ligands crucial for neonatal nervous system development. Anti-CNTF antibody (C-12) serves as an invaluable tool for studying CNTF′s role in both normal physiology and disease states.

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CNTF Antibody (C-12) References:

1. Expression of a fusion protein of human ciliary neurotrophic factor and soluble CNTF-receptor and identification of its activity.  |  Sun, Y., et al. 2003. J Zhejiang Univ Sci. 4: 340-5. PMID: 12765290
2. Activation of the ciliary neurotrophic factor (CNTF) signalling pathway in cortical neurons of multiple sclerosis patients.  |  Dutta, R., et al. 2007. Brain. 130: 2566-76. PMID: 17898009
3. Detection of ligand-induced CNTF receptor dimers in living cells by fluorescence cross correlation spectroscopy.  |  Neugart, F., et al. 2009. Biochim Biophys Acta. 1788: 1890-900. PMID: 19482006
4. The amino acid exchange R28E in ciliary neurotrophic factor (CNTF) abrogates interleukin-6 receptor-dependent but retains CNTF receptor-dependent signaling via glycoprotein 130 (gp130)/leukemia inhibitory factor receptor (LIFR).  |  Wagener, EM., et al. 2014. J Biol Chem. 289: 18442-50. PMID: 24802752
5. Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments.  |  Li, S., et al. 2018. J Biol Chem. 293: 15256-15268. PMID: 30115683
6. Ciliary neurotrophic factor (CNTF) and its receptor (CNTFRα) signal through MAPK/ERK pathway in human prostate tissues: a morphological and biomolecular study.  |  Fantone, S., et al. 2020. Eur J Histochem. 64: PMID: 33131268
7. CNTF variants with increased biological potency and receptor selectivity define a functional site of receptor interaction.  |  Saggio, I., et al. 1995. EMBO J. 14: 3045-54. PMID: 7621819
8. CNTF protection of oligodendrocytes against natural and tumor necrosis factor-induced death.  |  Louis, JC., et al. 1993. Science. 259: 689-92. PMID: 8430320
9. Ciliary neurotrophic factor (CNTF) genotypes and CNTF contents in human sciatic nerves as measured by a sensitive enzyme-linked immunoassay.  |  Takahashi, R., et al. 1996. J Neurochem. 67: 525-9. PMID: 8764576
10. Endogenous NGF and CNTF levels in human peripheral nerve injury.  |  Anand, P., et al. 1997. Neuroreport. 8: 1935-8. PMID: 9223080

The preferred CNTF antibody is CNTF Antibody (A-11): sc-25286.