CNPase Antibody (G-6): sc-166063

CNPase Antibody (G-6) is a mouse monoclonal IgG2a antibody that detects 2′,3′-cyclic nucleotide-3′-phosphodiesterase (CNPase) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CNPase plays a crucial role as a microtubule-associated protein, facilitating the assembly of microtubules, which are essential for maintaining cellular structure and function. The C-terminus of CNPase is particularly important for microtubule-promoting activity, highlighting CNPase′s significance in cytoskeletal dynamics. CNPase is predominantly located in the central nervous system, found in high concentrations within myelin and the outer segments of photoreceptors in the retina, underscoring CNPase′s vital role in neuronal health and visual function. Disruptions in CNPase activity, such as those caused by acute lead intoxication, can lead to detrimental effects on myelin morphology, further emphasizing CNPase′s importance in maintaining neural tissue integrity. Anti-CNPase antibody (G-6) is an invaluable tool for researchers studying the intricate roles of microtubules and myelin in various physiological and pathological contexts.

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CNPase Antibody (G-6) References:

1. Expression of the non-compact myelin protein 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) in olfactory bulb ensheathing glia from explant cultures.  |  Santos-Silva, A. and Cavalcante, LA. 2001. Neurosci Res. 40: 189-93. PMID: 11377758
2. CNPase activity in myelin from adult rat brains after prolonged lead exposure in vivo.  |  Dabrowska-Bouta, B., et al. 2004. Chem Biol Interact. 150: 171-8. PMID: 15535987
3. Activity of 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) in spinal cord with spongy change induced by a single oral dose of aniline in rats.  |  Kanno, T., et al. 2010. Toxicol Pathol. 38: 359-65. PMID: 20233944
4. Effect of lipid peroxidation on Na+,K(+)-ATPase, 5'-nucleotidase and CNPase in mouse brain myelin.  |  Domanska-Janik, K. and Bourre, JM. 1990. Biochim Biophys Acta. 1034: 200-6. PMID: 2162209
5. Enriched environment increases the total number of CNPase positive cells in the corpus callosum of middle-aged rats.  |  Zhao, YY., et al. 2011. Acta Neurobiol Exp (Wars). 71: 322-30. PMID: 22068741
6. Downregulation of CNPase in a MeCP2 deficient mouse model of Rett syndrome.  |  Wu, W., et al. 2012. Neurol Res. 34: 107-13. PMID: 22334035
7. Identification of phosphorylated form of 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) as 46 kDa phosphoprotein in brain non-synaptic mitochondria overloaded by calcium.  |  Azarashvili, T., et al. 2014. J Bioenerg Biomembr. 46: 135-45. PMID: 24532135
8. The promoter region of 46-kDa CNPase is sufficient for its expression in corpus callosum.  |  Miyamoto, Y., et al. 2018. Mol Genet Metab Rep. 15: 78-79. PMID: 29560317
9. Evaluation of CNPase and TGFβ1/Smad Signalling Pathway Molecule Expression in Sinus Epithelial Tissues of Patients with Chronic Rhinosinusitis with (CRSwNP) and without Nasal Polyps (CRSsNP).  |  Piszczatowska, K., et al. 2024. J Pers Med. 14: PMID: 39338148
10. CNPase expression in the developing opossum brain stem and cerebellum.  |  Barradas, PC., et al. 1995. Neuroreport. 6: 289-92. PMID: 7756612

The preferred CNPase antibody is CNPase Antibody (H-2): sc-166558.