CNOT6 CRISPR/Cas9 KO Plasmid (h): sc-406273
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CNOT6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CNOT6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CNOT6 Antibody (2193C2a): sc-81231
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CNOT6 CRISPR/Cas9 KO Plasmid (h) | sc-406273 | 20 µg | $397.00 |
Overview
CNOT6 (CCR4-NOT transcription complex subunit 6) is a catalytic deadenylase within the CCR4–NOT complex that shortens mRNA poly(A) tails to initiate transcript decay and tune translation. By regulating mRNA stability, CNOT6 contributes to post-transcriptional gene control across pathways governing cell-cycle progression, stress responses, differentiation, and innate immune signaling. Its activity interfaces with miRNA-mediated silencing and broader RNA surveillance networks, shaping transcriptome dynamics in response to cellular cues. Dysregulation of CCR4–NOT-dependent deadenylation, including altered CNOT6 function or expression, has been associated with aberrant gene-expression programs relevant to cancer biology and immune-related phenotypes.
CNOT6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CNOT6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CNOT6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CNOT6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CNOT6 protein expression.
This CRISPR knockout system enables efficient generation of CNOT6-deficient cell models for investigation of CNOT6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CNOT6 exon(s) critical for CNOT6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CNOT6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CNOT6 CRISPR/Cas9 KO Plasmid (h) and CNOT6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CNOT6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CNOT6 HDR Plasmid (h) and CNOT6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CNOT6 homology arms to support homology-directed repair at defined CNOT6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.