CNOT6 Antibody (2193C2a): sc-81231
- CNOT6 Antibody (2193C2a) is a mouse monoclonal IgG2b CNOT6 antibody, cited in 3 publications, provided at 100 µg/ml
- raised against a recombinant protein corresponding to the C-terminal region of CNOT6 of human origin
- recommended for detection of CNOT6 of human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CNOT6 Antibody (2193C2a). This work is in progress.
CNOT6 Antibody (2193C2a) is a mouse monoclonal IgG2b antibody that detects CNOT6 protein of human origin by western blotting (WB) and immunoprecipitation (IP). Anti-CNOT6 antibody (2193C2a) is available as a non-conjugated format, specifically designed to target CNOT6 protein, which is a crucial component of the CCR4-NOT transcription complex. This complex plays a vital role in regulating gene expression and mRNA degradation, making CNOT6 essential for maintaining cellular homeostasis and responding to environmental changes. CNOT6, belonging to the CCR4/nocturin family, contains three leucine-rich repeats, which are important for protein-protein interactions and stability. In the cytoplasm, CNOT6 functions as a poly(A) nuclease, facilitating the decay of mRNA, particularly through interaction with the major-protein-coding determinant of instability (mCRD) of the Fos gene. This process is critical for controlling the levels of specific mRNAs, thereby influencing various cellular processes such as differentiation, proliferation, and apoptosis. CNOT6′s ability to modulate mRNA stability highlights significance in post-transcriptional regulation and potential implications in diseases where mRNA turnover is disrupted.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CNOT6 Antibody (2193C2a) References:
- Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits. | Albert, TK., et al. 2000. Nucleic Acids Res. 28: 809-17. PMID: 10637334
- Identification of four families of yCCR4- and Mg2+-dependent endonuclease-related proteins in higher eukaryotes, and characterization of orthologs of yCCR4 with a conserved leucine-rich repeat essential for hCAF1/hPOP2 binding. | Dupressoir, A., et al. 2001. BMC Genomics. 2: 9. PMID: 11747467
- CCR4, a 3'-5' poly(A) RNA and ssDNA exonuclease, is the catalytic component of the cytoplasmic deadenylase. | Chen, J., et al. 2002. EMBO J. 21: 1414-26. PMID: 11889047
- Smaug recruits the CCR4/POP2/NOT deadenylase complex to trigger maternal transcript localization in the early Drosophila embryo. | Semotok, JL., et al. 2005. Curr Biol. 15: 284-94. PMID: 15723788
- Transcriptome analysis of human gastric cancer. | Oh, JH., et al. 2005. Mamm Genome. 16: 942-54. PMID: 16341674
- mRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes. | Behm-Ansmant, I., et al. 2006. Genes Dev. 20: 1885-98. PMID: 16815998
- Increased number of CCR4-positive cells in the duodenum of ovalbumin-induced food allergy model Nc/jic mice and antiallergic activity of fructooligosaccharides. | Fujitani, S., et al. 2007. Allergol Int. 56: 131-8. PMID: 17384533
- CCR4/NOT complex associates with the proteasome and regulates histone methylation. | Laribee, RN., et al. 2007. Proc Natl Acad Sci U S A. 104: 5836-41. PMID: 17389396
- Components of the CCR4-NOT complex function as nuclear hormone receptor coactivators via association with the NRC-interacting Factor NIF-1. | Garapaty, S., et al. 2008. J Biol Chem. 283: 6806-16. PMID: 18180299
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CNOT6 Antibody (2193C2a) | sc-81231 | 100 µg/ml | $333.00 |