CMAS Antibody (E-8): sc-398296
- CMAS Antibody (E-8) is a mouse monoclonal IgG1 κ CMAS antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 150-163 within an internal region of CMAS of human origin
- CMAS Antibody (E-8) is recommended for detection of CMAS of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-CMAS Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CMAS Antibody (E-8) for WB applications. These reagents are now offered in bundles with CMAS Antibody (E-8) (see ordering information below).
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CMAS Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects CMAS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CMAS (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CMAS, also known as cytidine monophosphate N-acetylneuraminic acid synthetase or CMP-sialic acid synthetase, is a crucial 434 amino acid enzyme that plays a significant role in sialic acid metabolism. CMAS is primarily located in the nucleus, where CMAS functions as a homotetramer, catalyzing the conversion of N-acetylneuraminic acid and cytidine triphosphate (CTP) into cytidine 5′-monophosphate N-acetylneuraminic acid (CMP-NeuNAc). CMP-NeuNAc serves as a donor substrate for sialyltransferases, which are responsible for adding sialic acid to the terminal ends of glycoproteins, polysaccharides, and glycolipids. This post-translational modification is vital for proper development, structure, and function of animal tissues, influencing various biological processes such as cell signaling, immune response, and cellular interactions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CMAS Antibody (E-8) References:
- Nuclear localization signal of murine CMP-Neu5Ac synthetase includes residues required for both nuclear targeting and enzymatic activity. | Munster, AK., et al. 2002. J Biol Chem. 277: 19688-96. PMID: 11893746
- High-level expression of recombinant Neisseria CMP-sialic acid synthetase in Escherichia coli. | Karwaski, MF., et al. 2002. Protein Expr Purif. 25: 237-40. PMID: 12135555
- Expression of a functional Drosophila melanogaster CMP-sialic acid synthetase. Differential localization of the Drosophila and human enzymes. | Viswanathan, K., et al. 2006. J Biol Chem. 281: 15929-40. PMID: 16537535
- The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme. | Tiralongo, J., et al. 2007. Glycobiology. 17: 945-54. PMID: 17580313
- Cloning and characterization of a heat-stable CMP-N-acylneuraminic acid synthetase from Clostridium thermocellum. | Mizanur, RM. and Pohl, NL. 2007. Appl Microbiol Biotechnol. 76: 827-34. PMID: 17602221
- Construction of a functional CMP-sialic acid biosynthesis pathway in Arabidopsis. | Castilho, A., et al. 2008. Plant Physiol. 147: 331-9. PMID: 18326787
- Mammalian cytidine 5'-monophosphate N-acetylneuraminic acid synthetase: a nuclear protein with evolutionarily conserved structural motifs. | Münster, AK., et al. 1998. Proc Natl Acad Sci U S A. 95: 9140-5. PMID: 9689047
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CMAS Antibody (E-8) | sc-398296 | 200 µg/ml | $322.00 | |||
CMAS Antibody (E-8): m-IgG Fc BP-HRP Bundle | sc-530744 | 200 µg Ab; 10 µg BP | $361.00 | |||
CMAS Antibody (E-8): m-IgGκ BP-HRP Bundle | sc-524279 | 200 µg Ab, 40 µg BP | $361.00 | |||
CMAS Antibody (E-8): m-IgG1 BP-HRP Bundle | sc-544231 | 200 µg Ab; 20 µg BP | $361.00 | |||
CMAS Antibody (E-8) AC | sc-398296 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CMAS Antibody (E-8) HRP | sc-398296 HRP | 200 µg/ml | $322.00 | |||
CMAS Antibody (E-8) FITC | sc-398296 FITC | 200 µg/ml | $336.00 | |||
CMAS Antibody (E-8) PE | sc-398296 PE | 200 µg/ml | $349.00 | |||
CMAS Antibody (E-8) Alexa Fluor® 488 | sc-398296 AF488 | 200 µg/ml | $364.00 | |||
CMAS Antibody (E-8) Alexa Fluor® 546 | sc-398296 AF546 | 200 µg/ml | $364.00 | |||
CMAS Antibody (E-8) Alexa Fluor® 594 | sc-398296 AF594 | 200 µg/ml | $364.00 | |||
CMAS Antibody (E-8) Alexa Fluor® 647 | sc-398296 AF647 | 200 µg/ml | $364.00 | |||
CMAS Antibody (E-8) Alexa Fluor® 680 | sc-398296 AF680 | 200 µg/ml | $364.00 | |||
CMAS Antibody (E-8) Alexa Fluor® 790 | sc-398296 AF790 | 200 µg/ml | $364.00 | |||
CMAS (E-8) Neutralizing Peptide | sc-398296 P | 100 µg/0.5 ml | $69.00 |