CLIM-2 Antibody (A-4): sc-514035

CLIM-2 Antibody (A-4) is a mouse monoclonal IgG1 antibody that detects CLIM-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CLIM-2, also known as nuclear LIM interactor, plays a crucial role in assembling transcriptional regulatory complexes essential for mediating gene expression. CLIM-2 interacts with LIM-only proteins, such as LMO2, to form heteromeric complexes that enhance stability and nuclear retention of LIM proteins, thereby influencing cellular differentiation and function. CLIM-2 regulates gene expression in various cell types, particularly in immune system and neural development. By facilitating complex formation, CLIM-2 contributes to precise control of transcriptional programs vital for proper cellular responses and developmental processes. Anti-CLIM-2 antibody (A-4) serves as an invaluable tool for researchers studying intricate mechanisms of gene regulation and roles of LIM domain proteins in health and disease.

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CLIM-2 Antibody (A-4) References:

1. Tandem LIM domains provide synergistic binding in the LMO4:Ldb1 complex.  |  Deane, JE., et al. 2004. EMBO J. 23: 3589-98. PMID: 15343268
2. Identification of the key LMO2-binding determinants on Ldb1.  |  Ryan, DP., et al. 2006. J Mol Biol. 359: 66-75. PMID: 16616188
3. The Ldb1 and Ldb2 transcriptional cofactors interact with the Ste20-like kinase SLK and regulate cell migration.  |  Storbeck, CJ., et al. 2009. Mol Biol Cell. 20: 4174-82. PMID: 19675209
4. Ldb1, a new guardian of hematopoietic stem cell maintenance.  |  Welinder, E. and Murre, C. 2011. Nat Immunol. 12: 113-4. PMID: 21245899
5. Ldb1-nucleated transcription complexes function as primary mediators of global erythroid gene activation.  |  Li, L., et al. 2013. Blood. 121: 4575-85. PMID: 23610375
6. Enhancer-bound LDB1 regulates a corticotrope promoter-pausing repression program.  |  Zhang, F., et al. 2015. Proc Natl Acad Sci U S A. 112: 1380-5. PMID: 25605944
7. The Isl1/Ldb1 Complex Orchestrates Genome-wide Chromatin Organization to Instruct Differentiation of Multipotent Cardiac Progenitors.  |  Caputo, L., et al. 2015. Cell Stem Cell. 17: 287-99. PMID: 26321200
8. SSBP3 Interacts With Islet-1 and Ldb1 to Impact Pancreatic β-Cell Target Genes.  |  Galloway, JR., et al. 2015. Mol Endocrinol. 29: 1774-86. PMID: 26495868
9. The stage-dependent roles of Ldb1 and functional redundancy with Ldb2 in mammalian retinogenesis.  |  Gueta, K., et al. 2016. Development. 143: 4182-4192. PMID: 27697904
10. LIM-Only Protein 4 (LMO4) and LIM Domain Binding Protein 1 (LDB1) Promote Growth and Metastasis of Human Head and Neck Cancer (LMO4 and LDB1 in Head and Neck Cancer).  |  Simonik, EA., et al. 2016. PLoS One. 11: e0164804. PMID: 27780223

The preferred CLIM-2 antibody is CLIM-2 Antibody (C-9): sc-365074.