CLIM-1/2 Antibody (A-3): sc-376030
- CLIM-1/2 Antibody (A-3) is a mouse monoclonal IgG2b κ CLIM-1/2 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminal of CLIM-1 of human origin
- CLIM-1/2 Antibody (A-3) is recommended for detection of CLIM-1 and CLIM-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine and canine
- Anti-CLIM-1/2 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-376030 X, 200 µg/0.1 ml)
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CLIM-1/2 Antibody (A-3) for WB and IHC(P) applications. These reagents are now offered in bundles with CLIM-1/2 Antibody (A-3) (see ordering information below).
CLIM-1/2 Antibody (A-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects CLIM-1/2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CLIM-1/2 (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CLIM-1/2, also known as LIM domain-binding factor-2, LDB1, CLP36, ELFIN, and CLP-36, is a crucial LIM domain-containing protein that plays a significant role in cellular signaling and transcriptional regulation. CLIM-1 is primarily located in the cytoplasm and interacts with various transcription factors, facilitating the formation of transcriptional complexes essential for gene expression. CLIM-1′s location enables modulation of transcription factor activity in response to cellular signals, influencing processes such as muscle development and cellular differentiation. CLIM-1 localizes to actin stress fibers in nonmuscle cells and associates with alpha-actinin through PDZ domain, highlighting a role in cytoskeletal organization and cellular architecture. CLIM-1 expression is most abundant in heart and skeletal muscle, as shown by northern blot analysis revealing an approximately 2.0-kb transcript, underscoring relevance in muscle physiology and potential implications in muscle-related diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CLIM-1/2 Antibody (A-3) References:
- The Ldb1 and Ldb2 transcriptional cofactors interact with the Ste20-like kinase SLK and regulate cell migration. | Storbeck, CJ., et al. 2009. Mol Biol Cell. 20: 4174-82. PMID: 19675209
- The stage-dependent roles of Ldb1 and functional redundancy with Ldb2 in mammalian retinogenesis. | Gueta, K., et al. 2016. Development. 143: 4182-4192. PMID: 27697904
- LDB1 Regulates Energy Homeostasis During Diet-Induced Obesity. | Loyd, C., et al. 2017. Endocrinology. 158: 1289-1297. PMID: 28009534
- LDB2 regulates the expression of DLL4 through the formation of oligomeric complexes in endothelial cells. | Choi, HJ., et al. 2018. BMB Rep. 51: 21-26. PMID: 28946938
- The LIM domain binding protein 1, Ldb1, has distinct roles in Neu-induced mammary tumorigenesis. | Ahmed, S., et al. 2018. Biochim Biophys Acta Mol Cell Res. 1865: 1590-1597. PMID: 30327200
- Crystal structure of human LDB1 in complex with SSBP2. | Wang, H., et al. 2020. Proc Natl Acad Sci U S A. 117: 1042-1048. PMID: 31892537
- Cooperation of LIM domain-binding 2 (LDB2) with EGR in the pathogenesis of schizophrenia. | Ohnishi, T., et al. 2021. EMBO Mol Med. 13: e12574. PMID: 33656268
- The transcriptional co-regulator LDB1 is required for brown adipose function. | Kepple, JD., et al. 2021. Mol Metab. 53: 101284. PMID: 34198011
- LMO2 promotes the development of AML through interaction with transcription co-regulator LDB1. | Lu, L., et al. 2023. Cell Death Dis. 14: 518. PMID: 37573405
- Enhancer looping protein LDB1 modulates MYB expression in T-ALL cell lines in vitro by cooperating with master transcription factors. | Li, Y., et al. 2024. J Exp Clin Cancer Res. 43: 283. PMID: 39385230
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLIM-1/2 Antibody (A-3) | sc-376030 | 200 µg/ml | $322.00 | |||
CLIM-1/2 Antibody (A-3): m-IgGκ BP-HRP Bundle | sc-522826 | 200 µg Ab, 40 µg BP | $361.00 | |||
CLIM-1/2 Antibody (A-3): m-IgG2b BP-HRP Bundle | sc-549074 | 200 µg Ab; 10 µg BP | $361.00 | |||
CLIM-1/2 Antibody (A-3) X | sc-376030 X | 200 µg/0.1 ml | $322.00 | |||
CLIM-1/2 Antibody (A-3) AC | sc-376030 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CLIM-1/2 Antibody (A-3) HRP | sc-376030 HRP | 200 µg/ml | $322.00 | |||
CLIM-1/2 Antibody (A-3) FITC | sc-376030 FITC | 200 µg/ml | $336.00 | |||
CLIM-1/2 Antibody (A-3) PE | sc-376030 PE | 200 µg/ml | $349.00 | |||
CLIM-1/2 Antibody (A-3) Alexa Fluor® 488 | sc-376030 AF488 | 200 µg/ml | $364.00 | |||
CLIM-1/2 Antibody (A-3) Alexa Fluor® 546 | sc-376030 AF546 | 200 µg/ml | $364.00 | |||
CLIM-1/2 Antibody (A-3) Alexa Fluor® 594 | sc-376030 AF594 | 200 µg/ml | $364.00 | |||
CLIM-1/2 Antibody (A-3) Alexa Fluor® 647 | sc-376030 AF647 | 200 µg/ml | $364.00 | |||
CLIM-1/2 Antibody (A-3) Alexa Fluor® 680 | sc-376030 AF680 | 200 µg/ml | $364.00 | |||
CLIM-1/2 Antibody (A-3) Alexa Fluor® 790 | sc-376030 AF790 | 200 µg/ml | $364.00 |