CLIC4/5/6 Antibody (A-11): sc-271863
- CLIC4/5/6 Antibody (A-11) is a mouse monoclonal IgG2a κ CLIC4/5/6 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 181-250 mapping within an internal region of CLIC5 of human origin
- CLIC4/5/6 Antibody (A-11) is recommended for detection of CLIC4, CLIC5 and CLIC6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CLIC4/5/6 Antibody (A-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CLIC4/5/6 Antibody (A-11) for WB and IHC(P) applications. These reagents are now offered in bundles with CLIC4/5/6 Antibody (A-11) (see ordering information below).
CLIC4/5/6 Antibody (A-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects CLIC4/5/6 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-CLIC4/5/6 antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CLIC4, also known as chloride intracellular channel 4, is a 253 amino acid single-pass membrane protein that forms selective ion channels essential for maintaining cellular homeostasis and signaling. CLIC4 is expressed in various tissues, including the placenta, heart, skeletal muscle, and epithelial cells of the kidney, colon, and esophagus, with localization to both nucleus and cytoplasm highlighting multifunctional nature. CLIC4 facilitates chloride and other ion transport vital for cell volume regulation and electrical excitability, making CLIC4 significant in physiological functions. CLIC5 and CLIC6 have distinct roles, with CLIC5 involved in actin-based cytoskeletal structure assembly and maintenance, while CLIC6 regulates chloride ion transport in water-secreting cells. CLIC6 interaction with dopamine receptors emphasizes these proteins′ importance in various signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CLIC4/5/6 Antibody (A-11) References:
- A novel p64-related Cl- channel: subcellular distribution and nephron segment-specific expression. | Edwards, JC. 1999. Am J Physiol. 276: F398-408. PMID: 10070163
- Identification of a novel member of the chloride intracellular channel gene family (CLIC5) that associates with the actin cytoskeleton of placental microvilli. | Berryman, M. and Bretscher, A. 2000. Mol Biol Cell. 11: 1509-21. PMID: 10793131
- Challenging accepted ion channel biology: p64 and the CLIC family of putative intracellular anion channel proteins (Review). | Ashley, RH. 2003. Mol Membr Biol. 20: 1-11. PMID: 12745921
- Identification of a novel member of the CLIC family, CLIC6, mapping to 21q22.12. | Friedli, M., et al. 2003. Gene. 320: 31-40. PMID: 14597386
- Proteomic analysis of vascular endothelial growth factor-induced endothelial cell differentiation reveals a role for chloride intracellular channel 4 (CLIC4) in tubular morphogenesis. | Bohman, S., et al. 2005. J Biol Chem. 280: 42397-404. PMID: 16239224
- The chloride intracellular channel protein CLIC5 is expressed at high levels in hair cell stereocilia and is essential for normal inner ear function. | Gagnon, LH., et al. 2006. J Neurosci. 26: 10188-98. PMID: 17021174
- Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers. | Suh, KS., et al. 2007. Clin Cancer Res. 13: 121-31. PMID: 17200346
- CLIC4 mediates and is required for Ca2+-induced keratinocyte differentiation. | Suh, KS., et al. 2007. J Cell Sci. 120: 2631-40. PMID: 17636002
- Functional reconstitution of mammalian 'chloride intracellular channels' CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin. | Singh, H., et al. 2007. FEBS J. 274: 6306-16. PMID: 18028448
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLIC4/5/6 Antibody (A-11) | sc-271863 | 200 µg/ml | $322.00 | |||
CLIC4/5/6 Antibody (A-11): m-IgG Fc BP-HRP Bundle | sc-529273 | 200 µg Ab; 10 µg BP | $361.00 | |||
CLIC4/5/6 Antibody (A-11): m-IgGκ BP-HRP Bundle | sc-522081 | 200 µg Ab, 40 µg BP | $361.00 | |||
CLIC4/5/6 Antibody (A-11): m-IgG2a BP-HRP Bundle | sc-547288 | 200 µg Ab; 10 µg BP | $361.00 | |||
CLIC4/5/6 Antibody (A-11) AC | sc-271863 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CLIC4/5/6 Antibody (A-11) HRP | sc-271863 HRP | 200 µg/ml | $322.00 | |||
CLIC4/5/6 Antibody (A-11) FITC | sc-271863 FITC | 200 µg/ml | $336.00 | |||
CLIC4/5/6 Antibody (A-11) PE | sc-271863 PE | 200 µg/ml | $349.00 | |||
CLIC4/5/6 Antibody (A-11) Alexa Fluor® 488 | sc-271863 AF488 | 200 µg/ml | $364.00 | |||
CLIC4/5/6 Antibody (A-11) Alexa Fluor® 546 | sc-271863 AF546 | 200 µg/ml | $364.00 | |||
CLIC4/5/6 Antibody (A-11) Alexa Fluor® 594 | sc-271863 AF594 | 200 µg/ml | $364.00 | |||
CLIC4/5/6 Antibody (A-11) Alexa Fluor® 647 | sc-271863 AF647 | 200 µg/ml | $364.00 | |||
CLIC4/5/6 Antibody (A-11) Alexa Fluor® 680 | sc-271863 AF680 | 200 µg/ml | $364.00 | |||
CLIC4/5/6 Antibody (A-11) Alexa Fluor® 790 | sc-271863 AF790 | 200 µg/ml | $364.00 |