CLIC1 Antibody (356.1): sc-81873

CLIC1 Antibody (356.1) is a mouse monoclonal IgG2a kappa light chain antibody that detects CLIC1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CLIC1 (356.1) antibody is available in a non-conjugated form, providing flexibility for various experimental needs. Chloride intracellular channel 1 (CLIC1), also known as NCC27, is a crucial member of a highly conserved family of chloride ion channels that can exist in both soluble and integral membrane forms. CLIC1 is primarily located in the cytoplasm and can translocate to the plasma membrane, where CLIC1 plays a vital role in regulating chloride ion transport across cellular membranes. This transport is essential for maintaining cellular homeostasis, influencing processes such as cell volume regulation, signal transduction, and muscle contraction. Notably, CLIC1′s ability to form anion-selective channels is modulated by redox state; CLIC1 exists as a monomer in a reduced environment but dimerizes upon oxidation, facilitating channel formation in lipid bilayers and vesicles. Furthermore, CLIC1 is implicated in macrophage activation, and CLIC1 downregulation has been linked to impaired tumor necrosis factor-alpha release in response to β-Amyloid protein stimulation, highlighting potential involvement in neurodegenerative diseases like Alzheimer′s. Insulin regulation of CLIC1, which may induce subnuclear relocalization, further underscores significance in cellular signaling pathways.

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CLIC1 Antibody (356.1) References:

1. Crystal structure of a soluble form of the intracellular chloride ion channel CLIC1 (NCC27) at 1.4-A resolution.  |  Harrop, SJ., et al. 2001. J Biol Chem. 276: 44993-5000. PMID: 11551966
2. CLIC1 inserts from the aqueous phase into phospholipid membranes, where it functions as an anion channel.  |  Tulk, BM., et al. 2002. Am J Physiol Cell Physiol. 282: C1103-12. PMID: 11940526
3. Recombinant CLIC1 (NCC27) assembles in lipid bilayers via a pH-dependent two-state process to form chloride ion channels with identical characteristics to those observed in Chinese hamster ovary cells expressing CLIC1.  |  Warton, K., et al. 2002. J Biol Chem. 277: 26003-11. PMID: 11978800
4. The intracellular chloride ion channel protein CLIC1 undergoes a redox-controlled structural transition.  |  Littler, DR., et al. 2004. J Biol Chem. 279: 9298-305. PMID: 14613939
5. Identification of chloride intracellular channel proteins in spermatozoa.  |  Myers, K., et al. 2004. FEBS Lett. 566: 136-40. PMID: 15147883
6. Involvement of the intracellular ion channel CLIC1 in microglia-mediated beta-amyloid-induced neurotoxicity.  |  Novarino, G., et al. 2004. J Neurosci. 24: 5322-30. PMID: 15190104
7. Proteomic analysis on insulin signaling in human hematopoietic cells: identification of CLIC1 and SRp20 as novel downstream effectors of insulin.  |  Saeki, K., et al. 2005. Am J Physiol Endocrinol Metab. 289: E419-28. PMID: 15827065
8. Mapping functional domains of chloride intracellular channel (CLIC) proteins in vivo.  |  Berry, KL. and Hobert, O. 2006. J Mol Biol. 359: 1316-33. PMID: 16737711
9. CLIC1 Drives Angiogenesis in Hepatocellular Carcinoma by Modulating VEGFA.  |  Wei, X., et al. 2022. Technol Cancer Res Treat. 21: 15330338221106820. PMID: 35722791
10. KCTD4 interacts with CLIC1 to disrupt calcium homeostasis and promote metastasis in esophageal cancer.  |  Zheng, C., et al. 2023. Acta Pharm Sin B. 13: 4217-4233. PMID: 37799381
11. CLIC1 regulation of cancer stem cells in glioblastoma.  |  Randhawa, K. and Jahani-Asl, A. 2023. Curr Top Membr. 92: 99-123. PMID: 38007271
12. A CLIC1 network coordinates matrix stiffness and the Warburg effect to promote tumor growth in pancreatic cancer.  |  Zheng, JH., et al. 2024. Cell Rep. 43: 114633. PMID: 39154343

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CLIC1 Antibody (356.1) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-CLIC1 Antibody (356.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CLIC1 Antibody (356.1) Specifications

Alternate Product Name: CLIC1

Product Clone Name: 356.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-242

Epitope Target: CLIC1

Epitope Species: human

Product Reactivity/Detection: CLIC1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for CLIC1 Antibody (356.1): N/A