cleaved Rock-1 Antibody (4H247): sc-71965

Cleaved Rock-1 Antibody (4H247) is a mouse monoclonal IgG kappa light chain antibody specifically designed to detect the cleaved form of ROCK1 (Rho-associated coiled-coil-containing protein kinase 1), targeting the caspase-3 cleavage site corresponding to amino acids 1113-1114 of human origin. ROCK1, also known as p160ROCK, ROKβ, or Rho-associated protein kinase 1, is a serine/threonine kinase that plays a pivotal role in regulating the actin cytoskeleton, cell motility, contraction, and apoptosis. Predominantly localized in the cytoplasm and associated with the actin cytoskeleton, ROCK1′s cellular location is critical for modulating stress fibers and focal adhesions, thereby influencing cell shape and movement. Upon activation by RhoA or cleavage by caspase-3 during apoptosis, ROCK1 can translocate to the plasma membrane, leading to increased phosphorylation of downstream targets and promoting cellular events such as membrane blebbing. ROCK1 (4H247) monoclonal antibody is reactive with the cleaved C-terminus of ROCK1 from mouse, rat, human, and canine origins and is suitable for detecting both the cleaved and full-length forms in western blotting (WB) and immunoprecipitation (IP) assays. ROCK1 (4H247) antibody aids in studying the molecular mechanisms underlying cytoskeletal dynamics and apoptosis, which are relevant in various physiological and pathological conditions, including cancer progression.

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cleaved Rock-1 Antibody (4H247) References:

1. Caspase-activated ROCK-1 allows erythroblast terminal maturation independently of cytokine-induced Rho signaling.  |  Gabet, AS., et al. 2011. Cell Death Differ. 18: 678-89. PMID: 21072057
2. The effect of ROCK-1 activity change on the adhesive and invasive ability of Y79 retinoblastoma cells.  |  Wang, J., et al. 2014. BMC Cancer. 14: 89. PMID: 24528629
3. Immunoexpression of ROCK-1 and MMP-9 as prognostic markers in breast cancer.  |  Bottino, J., et al. 2014. Acta Histochem. 116: 1367-73. PMID: 25218053
4. A novel proteolytic cleavage of ROCK 1 in cell death: Not only by caspases 3 and 7 but also by caspase 2.  |  Özdemir, A., et al. 2021. Biochem Biophys Res Commun. 547: 118-124. PMID: 33610039
5. RhoA/ROCK-1 Signaling Pathway and Oxidative Stress in Coronary Artery Disease Patients.  |  Dokumacioglu, E., et al. 2022. Braz J Cardiovasc Surg. 37: 212-218. PMID: 34236817
6. ROCK 1 and 2 affect the spatial architecture of 3D spheroids derived from human corneal stromal fibroblasts in different manners.  |  Ida, Y., et al. 2022. Sci Rep. 12: 7419. PMID: 35523828
7. Profilin 1 Induces Tumor Metastasis by Promoting Microvesicle Secretion Through the ROCK 1/p-MLC Pathway in Non-Small Cell Lung Cancer.  |  Wang, Y., et al. 2022. Front Pharmacol. 13: 890891. PMID: 35586060
8. Dexmedetomidine Attenuates Methotrexate-Induced Neurotoxicity and Memory Deficits in Rats through Improving Hippocampal Neurogenesis: The Role of miR-15a/ROCK-1/ERK1/2/CREB/BDNF Pathway Modulation.  |  Taha, M., et al. 2023. Int J Mol Sci. 24: PMID: 36614208
9. Connecting GSK-3β Inhibitory Activity with IKK-β or ROCK-1 Inhibition to Target Tau Aggregation and Neuroinflammation in Alzheimer's Disease-Discovery, In Vitro and In Cellulo Activity of Thiazole-Based Inhibitors.  |  Góral, I., et al. 2024. Molecules. 29: PMID: 38893493
10. The ROCK-1/2 inhibitor RKI-1447 blocks N-MYC, promotes cell death, and emerges as a synergistic partner for BET inhibitors in neuroblastoma.  |  Pepich, A., et al. 2024. Cancer Lett. 605: 217261. PMID: 39307412

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cleaved Rock-1 Antibody (4H247) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-cleaved Rock-1 Antibody (4H247) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cleaved Rock-1 Antibody (4H247) Specifications

Alternate Product Name: cleaved Rock-1

Product Clone Name: 4H247

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG kappa light chain

Amino Acids: 11131114

Epitope Target: Rock-1

Epitope Species: human

Product Reactivity/Detection: the cleaved C-terminus of Rock-1

Species Reactivity/Detection: mouse, rat, human, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 100 µg IgG kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for cleaved Rock-1 Antibody (4H247): N/A

The preferred Rock-1 antibody is Rock-1 Antibody (G-6): sc-17794.