cleaved Rock-1 Antibody (154C1465): sc-52953
- cleaved Rock-1 Antibody (154C1465) is a mouse monoclonal IgG, cited in 5 publications, provided at 100 µg/ml
- raised against the caspase-3 cleavage site (corresponding to amino acids 11131114) of Rock-1 of human origin
- recommended for detection of the cleaved C-terminus of Rock-1 of mouse, rat, human and canine origin by WB and IP; also recommended for detection of the full-length protein
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cleaved Rock-1 Antibody (154C1465). This work is in progress.
Cleaved Rock-1 Antibody (154C1465) is a mouse monoclonal IgG specifically designed to recognize the C-terminus of cleaved ROCK1 (Rho-associated coiled-coil-containing protein kinase 1). Anti-ROCK1 antibody (154C1465) targets the caspase-3 cleavage site corresponding to amino acids 1113-1114 of human ROCK1 and is reactive with human, mouse, rat, and canine samples. ROCK1, also known as p160ROCK or ROKβ, is a serine/threonine kinase integral to regulating the actin cytoskeleton, cell motility, contraction, and apoptosis. During apoptosis, caspase-3 cleaves ROCK1, resulting in a constitutively active form that enhances phosphorylation of substrates like myosin light chain (MLC), contributing to membrane blebbing, a hallmark of apoptotic cells. Anti-ROCK1 antibody (154C1465) is suitable for detecting both the cleaved and full-length forms of ROCK1 in western blotting (WB) and immunoprecipitation (IP) assays, making this reagent a valuable tool for studying apoptosis and cytoskeletal dynamics in various biological processes, including cancer progression and cell migration.
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cleaved Rock-1 Antibody (154C1465) References:
- Activation of Rho-associated coiled-coil protein kinase 1 (ROCK-1) by caspase-3 cleavage plays an essential role in cardiac myocyte apoptosis. | Chang, J., et al. 2006. Proc Natl Acad Sci U S A. 103: 14495-500. PMID: 16983089
- Ouabain-induced apoptosis and Rho kinase: a novel caspase-2 cleavage site and fragment of Rock-2. | Ark, M., et al. 2010. Apoptosis. 15: 1494-506. PMID: 20661774
- Hepatitis B vaccine induces apoptotic death in Hepa1-6 cells. | Hamza, H., et al. 2012. Apoptosis. 17: 516-27. PMID: 22249285
- Pharmacologic inhibition of ROCK2 suppresses amyloid-β production in an Alzheimer's disease mouse model. | Herskowitz, JH., et al. 2013. J Neurosci. 33: 19086-98. PMID: 24305806
- MicroRNA-582-5p Reduces Propofol-induced Apoptosis in Developing Neurons by Targeting ROCK1. | Zhang, Z., et al. 2020. Curr Neurovasc Res. 17: 140-146. PMID: 32031069
- MicroRNA-135a-5p Promotes the Functional Recovery of Spinal Cord Injury by Targeting SP1 and ROCK. | Wang, N., et al. 2020. Mol Ther Nucleic Acids. 22: 1063-1077. PMID: 33294293
- A novel proteolytic cleavage of ROCK 1 in cell death: Not only by caspases 3 and 7 but also by caspase 2. | Özdemir, A., et al. 2021. Biochem Biophys Res Commun. 547: 118-124. PMID: 33610039
- miR-148a-3p inhibits the proliferation and migration of bladder cancer via regulating the expression of ROCK-1. | Xu, C., et al. 2022. PeerJ. 10: e12724. PMID: 35127282
- RhoA, ROCK-1, and ROCK-2 Gene Expression and Polymorphisms in Cholesteatoma Patients. | Görür, K., et al. 2021. J Int Adv Otol. 17: 530-535. PMID: 35177391
- [Effect and mechanism of Poria cocos polysaccharides on myocardial cell apoptosis in rats with myocardial ischemia-reperfusion injury by regulating Rho-ROCK signaling pathway]. | Xie, J., et al. 2023. Zhongguo Zhong Yao Za Zhi. 48: 6434-6441. PMID: 38212001
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cleaved Rock-1 Antibody (154C1465) | sc-52953 | 100 µg/ml | $322.00 |