cleaved Rb Antibody (172C1094): sc-56217

Cleaved Rb Antibody (172C1094) is a mouse monoclonal IgG1 antibody that detects cleaved Rb in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). Cleaved Rb, also known as retinoblastoma protein (Rb or pRb), plays a crucial role in regulating the cell cycle, particularly at the G1/S transition, by interacting with transcription factors of the E2F family. Anti-cleaved Rb antibody (172C1094) enables researchers to study this regulation, which is vital for maintaining proper cell proliferation and differentiation, as mutations in the retinoblastoma tumor suppressor gene can lead to pediatric cancers such as retinoblastoma and other tumors. Cleaved Rb undergoes extensive post-translational modifications, including phosphorylation at multiple serine and threonine residues, which dictate activity and stability. For instance, phosphorylation of Rb at Ser 795 by cyclin D-dependent kinases marks a transition from a transcriptionally repressive state to an inactive, phosphorylated form, allowing for cell cycle progression. This dynamic regulation is essential for preventing uncontrolled cell division and tumorigenesis, making cleaved Rb a critical target for cancer research and therapeutic interventions. Cleaved Rb monoclonal antibody (172C1094) serves as an invaluable tool for studying the intricate mechanisms of cell cycle control and the implications of Rb dysregulation in cancer biology.

The preferred Rb antibody is Rb Antibody (IF8): sc-102.