claudin-1 Antibody (A-9): sc-166338

claudin-1 Antibody (A-9) is a mouse monoclonal IgG2b kappa light chain antibody targeting an epitope between amino acids 168-207 at the C-terminus of human claudin-1, with reactivity across human, mouse, rat, equine, canine, and bovine species by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Claudin-1, also known as CLDN1, is an essential member of the claudin family, which forms the backbone of tight junctions in epithelial and endothelial cells, regulating paracellular transport. Structurally, claudin-1 spans the membrane with four transmembrane domains and two extracellular loops, which are critical in forming tight junction strands that create selective barriers between cells. This unique structure allows claudin-1 to participate in maintaining cellular polarity and controlling ion permeability, vital functions in organs like the liver and kidney, where claudin-1 is most abundantly expressed. The structural configuration of claudin-1 also facilitates interactions with other tight junction proteins, such as occludins and junctional adhesion molecules, enabling complex tissue compartmentalization. Mutations in claudin-1 are linked to neonatal ichthyosis-sclerosing cholangitis syndrome, highlighting its role in tissue integrity. Anti-claudin-1 antibody (A-9) is available in both non-conjugated and multiple conjugated forms, making it versatile for research across various species and applications.

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claudin-1 Antibody (A-9) References:

1. Transmembrane proteins in the tight junction barrier.  |  Fanning, AS., et al. 1999. J Am Soc Nephrol. 10: 1337-45. PMID: 10361874
2. Clostridium perfringens enterotoxin binds to the second extracellular loop of claudin-3, a tight junction integral membrane protein.  |  Fujita, K., et al. 2000. FEBS Lett. 476: 258-61. PMID: 10913624
3. Heterogeneity in expression and subcellular localization of claudins 2, 3, 4, and 5 in the rat liver, pancreas, and gut.  |  Rahner, C., et al. 2001. Gastroenterology. 120: 411-22. PMID: 11159882
4. The roles of claudin superfamily proteins in paracellular transport.  |  Heiskala, M., et al. 2001. Traffic. 2: 93-8. PMID: 11247307
5. Molecular structure of tight junctions and their role in epithelial transport.  |  Anderson, JM. 2001. News Physiol Sci. 16: 126-30. PMID: 11443232
6. Interleukin-2 receptor beta subunit-dependent and -independent regulation of intestinal epithelial tight junctions.  |  Nishiyama, R., et al. 2001. J Biol Chem. 276: 35571-80. PMID: 11466322
7. Structural characterization of CD81-Claudin-1 hepatitis C virus receptor complexes.  |  Bonander, N., et al. 2011. Biochem Soc Trans. 39: 537-40. PMID: 21428935
8. [Claudin 1 as a target for anti-hepatitis C virus strategy].  |  Fukasawa, M. 2014. Yakugaku Zasshi. 134: 635-40. PMID: 24790046
9. Lubiprostone Induces Claudin-1 and Protects Intestinal Barrier Function.  |  Nishii, N., et al. 2020. Pharmacology. 105: 102-108. PMID: 31536982
10. Claudin-1-Targeted Nanoparticles for Delivery to Aging-Induced Alterations in the Blood-Brain Barrier.  |  Bony, BA., et al. 2021. ACS Nano. 15: 18520-18531. PMID: 34748307
11. The Role of Claudin-1 Expression in Follicular and Papillary Thyroid Neoplasm.  |  Miskad, UA., et al. 2022. Asian Pac J Cancer Prev. 23: 4023-4027. PMID: 36579982
12. Identification and characterization of a first-generation inhibitor of claudin-1 in colon cancer progression and metastasis.  |  Fatima, I., et al. 2023. Biomed Pharmacother. 159: 114255. PMID: 36696800

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-claudin-1 Antibody (A-9) has 91 citations in a variety of scientific publications.

Primary antibodies like Anti-claudin-1 Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

claudin-1 Antibody (A-9) Specifications

Alternate Product Name: claudin-1

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 168-207

Epitope Target: claudin-1

Epitope Species: human

Product Reactivity/Detection: claudin-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 91 citations in various scientific publications

Available Conjugates/Forms for claudin-1 Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred claudin-1 antibody is claudin-1 Antibody (XX7): sc-81796.