Date published: 2025-9-5
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claudin-1 Antibody (A-9) - Western Blotting - Image 384036
claudin-1 抗体 (A-9) HRP: sc-166338 HRP. 未经转染的 293T: sc-117752 (A), 转染人 claudin-1 的 293T: sc-113827 (B), SCC-4 (C), RT-4 (D) 和 T24 (E) 全细胞裂解液中 claudin-1 表达的直接 Western 印迹分析.
claudin-1 抗体 (A-9) HRP: sc-166338 HRP. 未经转染的 293T: sc-117752 (A), 转染人 claudin-1 的 293T: sc-113827 (B), SCC-4 (C), RT-4 (D) 和 T24 (E) 全细胞裂解液中 claudin-1 表达的直接 Western 印迹分析.

claudin-1 抗体 (A-9): sc-166338

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说明书
  • claudin-1 抗体 A-9 是小鼠单克隆 IgG2b κ，claudin-1抗体, 在91篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的claudin-1的C-terminus的氨基酸168-207之间
  • claudin-1 抗体 (A-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 claudin-1; 也和以下物种反应，包括： 和 equine, canine and bovine
  • 抗claudin-1抗体（A-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取claudin-1 (A-9): sc-166338的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 claudin-1 抗体 (A-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与claudin-1 抗体 (A-9) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Claudin 抗体包括 claudin-1, claudin-10, claudin-11, claudin-19, claudin-2, claudin-3, claudin-4, claudin-5, claudin-6 和 claudin-9

Claudin-1抗体（A-9）是一种IgG2b κ小鼠单克隆Claudin-1抗体（也称为CLDN1抗体），可通过WB、IP、IF、IHC(P)和ELISA方法检测小鼠、大鼠和人类的Claudin-1蛋白。Claudin-1抗体（A-9）既可以非偶联抗Claudin-1抗体的形式提供，也可以多种偶联形式的抗Claudin-1抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Claudin超家族由人类中许多结构相关的蛋白质组成。这些蛋白质是细胞旁转运中紧密连接的重要结构和功能组分。Claudins位于所有含紧密连接的组织中的上皮细胞和内皮细胞。已知有三类蛋白质定位于紧密连接，包括Claudins、Occludin和连接粘附分子。Claudins由四个跨膜结构域和两个细胞外环组成，构成紧密连接的链。Claudin的表达通常严格限制在不同组织的特定区域，并可能在通过紧密连接的跨细胞转运中发挥重要作用。Claudin-1是一种多次跨膜蛋白质，在肾脏和肝脏中高水平表达，在脾脏、心脏、大脑、肺和睾丸中表达水平较低。编码Claudin-1的基因缺陷是导致鱼鳞病-硬化性胆管炎新生儿综合征（NISCH）的原因，这是一种常染色体隐性综合征，其特征是普通型鱼鳞病、头皮毛发稀疏、瘢痕性脱发和硬化性胆管炎。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

claudin-1 抗体 (A-9) 参考文献:

  1. 紧密连接屏障中的跨膜蛋白  |  Fanning, AS., et al. 1999. J Am Soc Nephrol. 10: 1337-45. PMID: 10361874
  2. 产气荚膜梭状芽孢杆菌肠毒素能与紧密连接整体膜蛋白 Claudin-3 的第二个细胞外环结合。  |  Fujita, K., et al. 2000. FEBS Lett. 476: 258-61. PMID: 10913624
  3. 大鼠肝脏, 胰腺和肠道中克劳丁蛋白 2, 3, 4 和 5 的表达和亚细胞定域的异质性。  |  Rahner, C., et al. 2001. Gastroenterology. 120: 411-22. PMID: 11159882
  4. 克劳丁超家族蛋白在细胞外转运中的作用  |  Heiskala, M., et al. 2001. Traffic. 2: 93-8. PMID: 11247307
  5. 紧密连接的分子结构及其在上皮运输中的作用。  |  Anderson, JM. 2001. News Physiol Sci. 16: 126-30. PMID: 11443232
  6. 白细胞介素-2 受体 beta 亚基对肠上皮紧密连接的依赖性和非依赖性调控。  |  Nishiyama, R., et al. 2001. J Biol Chem. 276: 35571-80. PMID: 11466322
  7. 磷酸烯醇丙酮酸羧化酶1的Neddylation控制葡萄糖代谢。  |  Bonander, N., et al. 2011. Biochem Soc Trans. 39: 537-40. PMID: 21428935
  8. Lubiprostone诱导Claudin-1并保护肠屏障功能。  |  Fukasawa, M. 2014. Yakugaku Zasshi. 134: 635-40. PMID: 24790046
  9. CD81-Claudin-1丙型肝炎病毒受体复合物的结构特征。  |  Nishii, N., et al. 2020. Pharmacology. 105: 102-108. PMID: 31536982
  10. Claudin-1表达在甲状腺滤泡和乳头状甲状腺肿瘤中的作用。  |  Bony, BA., et al. 2021. ACS Nano. 15: 18520-18531. PMID: 34748307
  11. [Claudin 1作为抗丙型肝炎病毒策略的目标]。  |  Miskad, UA., et al. 2022. Asian Pac J Cancer Prev. 23: 4023-4027. PMID: 36579982
  12. 结肠癌进展和转移过程中 Claudin-1 的第一代抑制剂的鉴定和特征描述。  |  Fatima, I., et al. 2023. Biomed Pharmacother. 159: 114255. PMID: 36696800

订购信息

产品名称产品编号规格价格数量收藏夹

claudin-1 抗体 (A-9)

sc-166338
200 µg/ml
$316.00

claudin-1 (A-9): m-IgGκ BP-HRP 套装

sc-521568
200 µg Ab, 40 µg BP
$354.00

claudin-1 (A-9): m-IgG2b BP-HRP 套装

sc-548929
200 µg Ab; 10 µg BP
$354.00

claudin-1 抗体 (A-9) AC

sc-166338 AC
500 µg/ml, 25% agarose
$416.00

claudin-1 抗体 (A-9) HRP

sc-166338 HRP
200 µg/ml
$316.00

claudin-1 抗体 (A-9) FITC

sc-166338 FITC
200 µg/ml
$330.00

claudin-1 抗体 (A-9) PE

sc-166338 PE
200 µg/ml
$343.00

claudin-1 抗体 (A-9) Alexa Fluor® 488

sc-166338 AF488
200 µg/ml
$357.00

claudin-1 抗体 (A-9) Alexa Fluor® 546

sc-166338 AF546
200 µg/ml
$357.00

claudin-1 抗体 (A-9) Alexa Fluor® 594

sc-166338 AF594
200 µg/ml
$357.00

claudin-1 抗体 (A-9) Alexa Fluor® 647

sc-166338 AF647
200 µg/ml
$357.00

claudin-1 抗体 (A-9) Alexa Fluor® 680

sc-166338 AF680
200 µg/ml
$357.00

claudin-1 抗体 (A-9) Alexa Fluor® 790

sc-166338 AF790
200 µg/ml
$357.00

claudin-1 (A-9) 中和勝肽

sc-166338 P
100 µg/0.5 ml
$68.00

I want to perform IHC or IF of the skin section using a claudin-1 antibody (c-166338). However, I could not find the recommended protocol.Please provide the detailed protocol about antigen retrieval buffer, blocking buffer, washing buffer, dilution buffer

Asked by: daisy0128
Thank you for your question. You can find our protocols under this link: https://www.scbt.com/resources/protocols
Answered by: Tech Support Europe
Date published: 2021-04-21

can SC-166338 be biotinylated? I have an EZ-Link NHS-PEG 4-Biotin Biotinylation kit cat#21329

Asked by: Karen67
Thank you for your question. We are not familiar with that exact conjugation kit, however the standard formulation of this antibody does contain gelatin as a stabilizing protein, and the gelatin may interfere with the conjugation process for the antibody. We can offer this antibody without gelatin, or conjugated to Biotin, as a special order. Please contact our Technical Service Department for more information or to request a quote. You can also email specialorders@scbt.com.
Answered by: Technical Support
Date published: 2020-04-28

Hello! Is there possibility to receive one sample unit of sc-166338 to try it before we order a full version)? Thanks in advance

Asked by: Vadim_Dolgin
Thank you for your question. Please contact our Technical Service Department or your local Distributor to arrange for a free 10 µg sample of claudin-1 (A-9): sc-166338.
Answered by: Tech Support Europe
Date published: 2019-09-23

Can claudin-1 Antibody (A-9): sc-166338 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Anna99
Yes, claudin-1 Antibody (A-9): sc-166338 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 10
Date published: 2017-01-09
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Rated 5 out of 5 by from Good antibodyWorks as expected. Tested on by immunofluorescence.
Date published: 2018-12-18
Rated 5 out of 5 by from excellent bandI use this antibody for western blotting analysis and the results were very excellent!
Date published: 2018-06-11
Rated 5 out of 5 by from worked in IFThis antibody worked in IF for Caco-2 cells. methanol fix only
Date published: 2018-05-10
Rated 2 out of 5 by from IF - rat tissue sectionsTested a sample of this Ab on rat tissue sections; very faint staining and some background. It may work better with cells but I would not recommend it for tissue cryosections (the XX7 antibody works better).
Date published: 2018-02-20
Rated 4 out of 5 by from Great antibodyThis antibody has great affinity, and localizes the antigen very specifically.
Date published: 2018-02-14
Rated 4 out of 5 by from Good antibody in IF at 1:50.This antibody is usefull in cell tight junction in IF.
Date published: 2017-11-23
Rated 4 out of 5 by from goodcytoplasma of the cells got non-specific staining in IHC
Date published: 2017-10-31
Rated 4 out of 5 by from goodthe color is obvious in cell membranes, but the cytoplasm also get non-specific staining
Date published: 2017-10-31
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claudin-1 抗体 (A-9) is rated 4.6 out of 5 by 23.
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