Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Clathrin Light Chain B/CLTB CRISPR/Cas9 KO Plasmid (h): sc-404228
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Clathrin Light Chain B/CLTB CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Clathrin Light Chain B/CLTB genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Clathrin Light Chain B/CLTB Antibody (G-7): sc-376414
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Clathrin Light Chain B/CLTB CRISPR/Cas9 KO Plasmid (h) | sc-404228 | 20 µg | $397.00 |
Overview
CLTB encodes clathrin light chain B, a regulatory subunit of the clathrin triskelion that modulates coat assembly, lattice dynamics, and adaptor interactions during clathrin-mediated endocytosis. Through its role in vesicle formation at the plasma membrane and trans-Golgi network, CLTB supports receptor internalization, synaptic vesicle cycling, and sorting of membrane proteins that influence downstream signaling and nutrient uptake. CLTB-dependent trafficking interfaces with endosomal recycling and lysosomal routing, shaping receptor residence time and pathway sensitivity. Dysregulated clathrin coat function and endocytic flux are frequently linked to altered signal transduction and proteostasis phenotypes studied in cancer biology and neurodegeneration models.
Clathrin Light Chain B/CLTB CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CLTB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CLTB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CLTB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Clathrin Light Chain B/CLTB protein expression.
This CRISPR knockout system enables efficient generation of CLTB-deficient cell models for investigation of Clathrin Light Chain B/CLTB signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CLTB exon(s) critical for Clathrin Light Chain B/CLTB function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CLTB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Clathrin Light Chain B/CLTB CRISPR/Cas9 KO Plasmid (h) and Clathrin Light Chain B/CLTB CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CLTB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Clathrin Light Chain B/CLTB HDR Plasmid (h) and Clathrin Light Chain B/CLTB HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CLTB homology arms to support homology-directed repair at defined CLTB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.