Clathrin Light Chain Antibody (CON.1): sc-12735

Clathrin LC Antibody (CON.1) is a mouse monoclonal IgG2b kappa light chain antibody that detects Clathrin LC protein across multiple species through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Clathrin LC antibody (CON.1) comes in non-conjugated form and various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® options. Clathrin plays a crucial role in intracellular transport, particularly in receptor-mediated endocytosis, facilitating receptor protein engulfment into clathrin-coated vesicles. Clathrin LC protein resides primarily in the cytosol, assembling into a triskelion structure composed of three heavy chains and three light chains. This structural arrangement enables coated vesicle formation for protein and lipid transport within cells. Light chains, specifically Clathrin light chain A (LCA) and Clathrin light chain B (LCB), regulate triskelion self-assembly onto membranes, influencing vesicle formation efficiency. LCA and LCB, encoded by distinct genes sharing 60% homology, demonstrate similar functional characteristics, including alternative mRNA splicing for tissue-specific isoforms. Brain-specific light chain isoforms contain inserted sequences forming higher molecular weight variants, essential for neuronal function and signaling.

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Clathrin Light Chain Antibody (CON.1) References:

1. Neuron-specific expression of high-molecular-weight clathrin light chain.  |  Wong, DH., et al. 1990. J Neurosci. 10: 3025-31. PMID: 2204686
2. Predominance of clathrin light chain LCb correlates with the presence of a regulated secretory pathway.  |  Acton, SL. and Brodsky, FM. 1990. J Cell Biol. 111: 1419-26. PMID: 2211818
3. Structure and assembly of coated vesicles.  |  Pearse, BM. and Crowther, RA. 1987. Annu Rev Biophys Biophys Chem. 16: 49-68. PMID: 2885011
4. Clathrin and coated vesicles.  |  Pearse, BM. 1987. EMBO J. 6: 2507-12. PMID: 2890519
5. Structure of human clathrin light chains. Conservation of light chain polymorphism in three mammalian species.  |  Jackson, AP. and Parham, P. 1988. J Biol Chem. 263: 16688-95. PMID: 3267234
6. Clathrin light chain-conjugated drug delivery for cancer.  |  Jung, S., et al. 2023. Bioeng Transl Med. 8: e10273. PMID: 36684105
7. Clathrin light chain A-enriched small extracellular vesicles remodel microvascular niche to induce hepatocellular carcinoma metastasis.  |  Xu, Y., et al. 2023. J Extracell Vesicles. 12: e12359. PMID: 37606345
8. CLC2 (Clathrin Light Chain 2)-ATG8h/ATG8i interactions connect clathrin-mediated endocytosis (CME) and the autophagy pathway.  |  Lan, H., et al. 2025. Autophagy. 21: 246-248. PMID: 39396122
9. Chromosomal location and some structural features of human clathrin light-chain genes (CLTA and CLTB).  |  Ponnambalam, S., et al. 1994. Genomics. 24: 440-4. PMID: 7713494
10. The interaction of calmodulin with clathrin-coated vesicles, triskelions, and light chains. Localization of a binding site.  |  Pley, UM., et al. 1995. J Biol Chem. 270: 2395-402. PMID: 7836475
11. Involvement of clathrin light chains in the pathology of Alzheimer's disease.  |  Nakamura, Y., et al. 1994. Acta Neuropathol. 87: 23-31. PMID: 8140893
12. Alteration of clathrin light chain expression by transfection and gene disruption.  |  Acton, SL., et al. 1993. Mol Biol Cell. 4: 647-60. PMID: 8374173

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Clathrin Light Chain Antibody (CON.1) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Clathrin Light Chain Antibody (CON.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Clathrin Light Chain Antibody (CON.1) Specifications

Alternate Product Name: Clathrin LC

Product Clone Name: CON.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 23-44

Epitope Target: Clathrin LC

Epitope Species: bovine

Product Reactivity/Detection: Clathrin LCA and LCB

Species Reactivity/Detection: broad species

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Clathrin Light Chain Antibody (CON.1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin