CIP4 Antibody (F-10): sc-166810

CIP4 Antibody (F-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects CIP4 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-CIP4 antibody (F-10) is available in non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. CIP4, also known as Cdc42-interacting protein 4 or TRIP10, is a 601 amino acid protein that localizes to the cytoplasm, cytoskeleton, lysosome, and Golgi apparatus. CIP4 contains one FCH domain, one SH3 domain, and one REM repeat, which are essential for coordinating membrane tubulation with actin cytoskeletal reorganization during endocytosis. CIP4 is required for insulin-dependent translocation of Glut4 to the plasma membrane, highlighting its importance in glucose metabolism and cellular energy homeostasis. CIP4 exists as multiple alternatively spliced isoforms and undergoes post-translational modifications, including tyrosine phosphorylation, which influence activity and interactions with other proteins. Aberrant splicing of CIP4 has been linked to renal cell carcinoma pathogenesis, suggesting a significant role in tumor transformation and metastasis, making CIP4 a valuable target for cancer biology research.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CIP4 Antibody (F-10) References:

1. Identification and genetic analysis of human and mouse activated Cdc42 interacting protein-4 isoforms.  |  Wang, L., et al. 2002. Biochem Biophys Res Commun. 293: 1426-30. PMID: 12054674
2. Cdc42-interacting protein 4 binds to huntingtin: neuropathologic and biological evidence for a role in Huntington's disease.  |  Holbert, S., et al. 2003. Proc Natl Acad Sci U S A. 100: 2712-7. PMID: 12604778
3. AKAP350 interaction with cdc42 interacting protein 4 at the Golgi apparatus.  |  Larocca, MC., et al. 2004. Mol Biol Cell. 15: 2771-81. PMID: 15047863
4. Splicing variant of Cdc42 interacting protein-4 disrupts beta-catenin-mediated cell-cell adhesion: expression and function in renal cell carcinoma.  |  Tsuji, E., et al. 2006. Biochem Biophys Res Commun. 339: 1083-8. PMID: 16343437
5. Curved EFC/F-BAR-domain dimers are joined end to end into a filament for membrane invagination in endocytosis.  |  Shimada, A., et al. 2007. Cell. 129: 761-72. PMID: 17512409
6. Cdc42-interacting protein-4 functionally links actin and microtubule networks at the cytolytic NK cell immunological synapse.  |  Banerjee, PP., et al. 2007. J Exp Med. 204: 2305-20. PMID: 17785506
7. Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor.  |  Lee, JW., et al. 1995. Mol Endocrinol. 9: 243-54. PMID: 7776974
8. A Cdc42 target protein with homology to the non-kinase domain of FER has a potential role in regulating the actin cytoskeleton.  |  Aspenström, P. 1997. Curr Biol. 7: 479-87. PMID: 9210375

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CIP4 Antibody (F-10) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CIP4 Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CIP4 Antibody (F-10) Specifications

Alternate Product Name: CIP4

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 467-558

Epitope Target: CIP4

Epitope Species: human

Product Reactivity/Detection: CIP4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CIP4 Antibody (F-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin