Anti-CIP4 Antibody (F-10) recommended for detection of CIP4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-CIP4 Antibody (F-10): sc-166810

Anti-CIP4 Antibody (F-10) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-CIP4 Antibody (F-10) is a mouse monoclonal IgG2a κ CIP4 antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 467-558 mapping within an internal region of CIP4 of human origin
    • Anti-CIP4 Antibody (F-10) is recommended for detection of CIP4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-CIP4 Antibody (F-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CIP4 (F-10): sc-166810.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for CIP4 Antibody (F-10) for WB and IHC(P) applications. These reagents are now offered in bundles with CIP4 Antibody (F-10) (see ordering information below).
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Can CIP4 (F-10): sc-166810 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, CIP4 (F-10): sc-166810 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Great We asked a sample from Santa. It was highly specific in MDCK cells by IF.
Date published: 2017-10-07
Rated 5 out of 5 by from Published WB and IHC data using human breast Published WB and IHC data using human breast tumor tissue and cell lines.
Date published: 2015-07-15
Rated 5 out of 5 by from Great immunofluorescence cytoplasmic and Great immunofluorescence cytoplasmic and membrane staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-01-11
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