CIB Antibody (G-5): sc-271490

CIB Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects CIB protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CIB (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CIB protein, known for its role as a calcium- and integrin-binding protein, is crucial in regulating the function of Integrin α2b/β3 (GPIIb/IIIa), which serves as the platelet fibrinogen receptor, facilitating the binding of proteins to integrin cytoplasmic domains. The interaction of CIB with the cytoplasmic domain of α2b is mediated by positively charged residues, and this binding is sensitive to changes in intracellular calcium levels, highlighting CIB′s importance in platelet aggregation and signaling. CIB′s cellular localization is dynamic, as CIB translocates to the Triton X-100-insoluble cytoskeleton in aggregated platelets, indicating a regulatory role in cellular processes. Beyond integrin interaction, CIB also binds to DNA-PKcs, a nuclear protein serine/threonine kinase involved in DNA repair and lymphoid development, suggesting CIB may function as a regulatory subunit for polo-like kinases. With significant homology to calcineurin B and calmodulin, CIB mRNA is widely expressed across various human tissues, underscoring CIB′s potential relevance in diverse biological contexts.

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CIB Antibody (G-5) References:

1. Calcium-dependent properties of CIB binding to the integrin alphaIIb cytoplasmic domain and translocation to the platelet cytoskeleton.  |  Shock, DD., et al. 1999. Biochem J. 342 Pt 3: 729-35. PMID: 10477286
2. Structures of the platelet calcium- and integrin-binding protein and the alphaIIb-integrin cytoplasmic domain suggest a mechanism for calcium-regulated recognition; homology modelling and NMR studies.  |  Hwang, PM. and Vogel, HJ. 2000. J Mol Recognit. 13: 83-92. PMID: 10822252
3. Genomic structure of mouse and human genes for DNA-PKcs interacting protein (KIP).  |  Hattori, A., et al. 2000. DNA Seq. 10: 415-8. PMID: 10826701
4. Adhesion induced expression of the serine/threonine kinase Fnk in human macrophages.  |  Holtrich, U., et al. 2000. Oncogene. 19: 4832-9. PMID: 11039900
5. Recent molecular advances in studies of the concentrative Na+-dependent nucleoside transporter (CNT) family: identification and characterization of novel human and mouse proteins (hCNT3 and mCNT3) broadly selective for purine and pyrimidine nucleosides (system cib).  |  Ritzel, MW., et al. 2001. Mol Membr Biol. 18: 65-72. PMID: 11396613
6. Association of CIB with GPIIb/IIIa during outside-in signaling is required for platelet spreading on fibrinogen.  |  Naik, UP. and Naik, MU. 2003. Blood. 102: 1355-62. PMID: 12714504
7. Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase.  |  Taslimi, A., et al. 2016. Nat Chem Biol. 12: 425-30. PMID: 27065233
8. Enhancing tellurite and selenite bioconversions by overexpressing a methyltransferase from Aromatoleum sp. CIB.  |  Alonso-Fernandes, E., et al. 2023. Microb Biotechnol. 16: 915-930. PMID: 36366868
9. Penazaphilones J-L, Three New Hydrophilic Azaphilone Pigments from Penicillium sclerotiorum cib-411 and Their Anti-Inflammatory Activity.  |  Zhang, X., et al. 2023. Molecules. 28: PMID: 37049911
10. Identification of a novel calcium-binding protein that interacts with the integrin alphaIIb cytoplasmic domain.  |  Naik, UP., et al. 1997. J Biol Chem. 272: 4651-4. PMID: 9030514
11. Interaction between DNA-dependent protein kinase and a novel protein, KIP.  |  Wu, X. and Lieber, MR. 1997. Mutat Res. 385: 13-20. PMID: 9372844
12. Structure, expression profile and chromosomal location of an isolog of DNA-PKcs interacting protein (KIP) gene.  |  Seki, N., et al. 1999. Biochim Biophys Acta. 1444: 143-7. PMID: 9931475

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CIB Antibody (G-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-CIB Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CIB Antibody (G-5) Specifications

Alternate Product Name: CIB

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 77-191

Epitope Target: CIB

Epitope Species: human

Product Reactivity/Detection: CIB

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for CIB Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin