Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Chymotrypsin CRISPR/Cas9 KO Plasmid (h): sc-407305
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Chymotrypsin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Chymotrypsin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Chymotrypsin Antibody (602): sc-59483
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Chymotrypsin CRISPR/Cas9 KO Plasmid (h) | sc-407305 | 20 µg | $397.00 |
Overview
CTRC encodes chymotrypsin C, a secreted pancreatic serine protease that modulates digestive protease activity by processing trypsinogens and degrading active trypsin, thereby contributing to protease homeostasis in the exocrine pancreas. Through regulated proteolysis within the pancreatic enzyme activation network, CTRC helps shape downstream protein digestion and limits aberrant intrapancreatic trypsin activity. Dysregulated CTRC function and altered protease balance are implicated in pancreatic inflammatory phenotypes, including susceptibility to chronic pancreatitis and related exocrine dysfunction. CTRC is therefore studied in pathways controlling zymogen activation, endoplasmic reticulum stress responses in acinar cells, and protease-mediated tissue injury.
Chymotrypsin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CTRC gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CTRC together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CTRC open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Chymotrypsin protein expression.
This CRISPR knockout system enables efficient generation of CTRC-deficient cell models for investigation of Chymotrypsin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CTRC exon(s) critical for Chymotrypsin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CTRC genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Chymotrypsin CRISPR/Cas9 KO Plasmid (h) and Chymotrypsin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CTRC locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Chymotrypsin HDR Plasmid (h) and Chymotrypsin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CTRC homology arms to support homology-directed repair at defined CTRC target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.