CHPT1 Antibody (F-7): sc-515577
- CHPT1 Antibody (F-7) is a mouse monoclonal IgG2a κ CHPT1 antibody, cited in 5 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 17-33 near the N-terminus of CHPT1 of human origin
- CHPT1 Antibody (F-7) is recommended for detection of CHPT1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CHPT1 Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CHPT1 Antibody (F-7) for WB applications. These reagents are now offered in bundles with CHPT1 Antibody (F-7) (see ordering information below).
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CHPT1 Antibody (F-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects CHPT1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CHPT1 (F-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CHPT1 protein, also known as Cholinephosphotransferase 1, is a 406 amino acid multi-pass membrane protein primarily localized to the Golgi apparatus, where CHPT1 plays a crucial role in phosphatidylcholine biosynthesis from CDP-choline, a key component in vesicular membrane formation and maintenance. CHPT1 function is vital for cellular integrity and membrane dynamics, particularly in tissues with high membrane turnover, such as testis, small intestine, heart, colon, spleen, and prostate. CHPT1 expression is significantly elevated in cancerous breast cells compared to normal breast cell lines, and CHPT1 gene mutations have been identified in these cancerous cells, suggesting a potential role in tumorigenesis. Exposure to mustard gas markedly reduces CHPT1 gene expression and activity, which may contribute to acute respiratory distress syndrome (ARDS) pathophysiology. Two CHPT1 isoforms arise from alternative splicing, adding complexity to CHPT1 functional roles in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CHPT1 Antibody (F-7) References:
- Cloning and expression of a human choline/ethanolaminephosphotransferase: synthesis of phosphatidylcholine and phosphatidylethanolamine. | Henneberry, AL. and McMaster, CR. 1999. Biochem J. 339 (Pt 2): 291-8. PMID: 10191259
- Cloning, genomic organization, and characterization of a human cholinephosphotransferase. | Henneberry, AL., et al. 2000. J Biol Chem. 275: 29808-15. PMID: 10893425
- Effect of ceramides on phospholipid biosynthesis and its implication for apoptosis. | Vaandrager, AB. and Houweling, M. 2002. Subcell Biochem. 36: 207-27. PMID: 12037983
- Differential expression of cholinephosphotransferase in normal and cancerous human mammary epithelial cells. | Ghosh, A., et al. 2002. Biochem Biophys Res Commun. 297: 1043-8. PMID: 12359261
- Phosphatidylcholine and cell death. | Cui, Z. and Houweling, M. 2002. Biochim Biophys Acta. 1585: 87-96. PMID: 12531541
- Inhibition of cholinephosphotransferase activity in lung injury induced by 2-chloroethyl ethyl sulfide, a mustard analog. | Sinha Roy, S., et al. 2005. J Biochem Mol Toxicol. 19: 289-97. PMID: 16292752
- Phosphatidylcholine metabolism during renal growth and regeneration. | Toback, FG. 1984. Am J Physiol. 246: F249-59. PMID: 6367486
- CDP-choline:1,2-diacylglycerol cholinephosphotransferase. | McMaster, CR. and Bell, RM. 1997. Biochim Biophys Acta. 1348: 100-10. PMID: 9370321
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHPT1 Antibody (F-7) | sc-515577 | 200 µg/ml | $322.00 | |||
CHPT1 Antibody (F-7): m-IgG Fc BP-HRP Bundle | sc-531424 | 200 µg Ab; 10 µg BP | $361.00 | |||
CHPT1 Antibody (F-7): m-IgGκ BP-HRP Bundle | sc-525112 | 200 µg Ab, 40 µg BP | $361.00 | |||
CHPT1 Antibody (F-7): m-IgG2a BP-HRP Bundle | sc-547787 | 200 µg Ab; 10 µg BP | $361.00 | |||
CHPT1 Antibody (F-7) AC | sc-515577 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CHPT1 Antibody (F-7) HRP | sc-515577 HRP | 200 µg/ml | $322.00 | |||
CHPT1 Antibody (F-7) FITC | sc-515577 FITC | 200 µg/ml | $336.00 | |||
CHPT1 Antibody (F-7) PE | sc-515577 PE | 200 µg/ml | $349.00 | |||
CHPT1 Antibody (F-7) Alexa Fluor® 488 | sc-515577 AF488 | 200 µg/ml | $364.00 | |||
CHPT1 Antibody (F-7) Alexa Fluor® 546 | sc-515577 AF546 | 200 µg/ml | $364.00 | |||
CHPT1 Antibody (F-7) Alexa Fluor® 594 | sc-515577 AF594 | 200 µg/ml | $364.00 | |||
CHPT1 Antibody (F-7) Alexa Fluor® 647 | sc-515577 AF647 | 200 µg/ml | $364.00 | |||
CHPT1 Antibody (F-7) Alexa Fluor® 680 | sc-515577 AF680 | 200 µg/ml | $364.00 | |||
CHPT1 Antibody (F-7) Alexa Fluor® 790 | sc-515577 AF790 | 200 µg/ml | $364.00 | |||
CHPT1 (F-7) Neutralizing Peptide | sc-515577 P | 100 µg/0.5 ml | $69.00 |