ChoK Antibody (B-8): sc-376489
- ChoK Antibody (B-8) is a mouse monoclonal IgG1 κ ChoK antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 91-300 mapping within an internal region of ChoKα of human origin
- ChoK Antibody (B-8) is recommended for detection of ChoKα isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ChoK Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ChoK Antibody (B-8) for WB applications. These reagents are now offered in bundles with ChoK Antibody (B-8) (see ordering information below).
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ChoK Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects ChoK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ChoK antibody (B-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ChoK plays a crucial role in the biosynthesis of phosphatidylcholine through the CDP-choline pathway, which is essential for cell membrane integrity and signaling. As the initial enzyme in this pathway, choline kinase is vital for cell growth and proliferation; ChoK activity is particularly important in cancer, where dysregulation can lead to increased levels of phosphorylcholine, a marker associated with tumorigenesis. Notably, ChoK inhibition by Hemicholinium-3 has been shown to significantly impede cell cycle progression in response to growth factors, highlighting ChoK′s potential as a therapeutic target. ChoK dysregulation has been implicated in various human cancers, including breast, lung, colorectal, and prostate tumors, making ChoK a significant focus of cancer research. The human choline kinase gene is located on chromosome 11q13.2, underscoring ChoK′s relevance in genetic studies related to cancer susceptibility.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ChoK Antibody (B-8) References:
- In vivo antitumor activity of choline kinase inhibitors: a novel target for anticancer drug discovery. | Hernández-Alcoceba, R., et al. 1999. Cancer Res. 59: 3112-8. PMID: 10397253
- Modulation of phospholipase D by hexadecylphosphorylcholine: a putative novel mechanism for its antitumoral activity. | Lucas, L., et al. 2001. Oncogene. 20: 1110-7. PMID: 11314048
- Increased choline kinase activity in human breast carcinomas: clinical evidence for a potential novel antitumor strategy. | Ramírez de Molina, A., et al. 2002. Oncogene. 21: 4317-22. PMID: 12082619
- Overexpression of choline kinase is a frequent feature in human tumor-derived cell lines and in lung, prostate, and colorectal human cancers. | Ramírez de Molina, A., et al. 2002. Biochem Biophys Res Commun. 296: 580-3. PMID: 12176020
- Generation of phosphorylcholine as an essential event in the activation of Raf-1 and MAP-kinases in growth factors-induced mitogenic stimulation. | Jiménez, B., et al. 1995. J Cell Biochem. 57: 141-9. PMID: 7721953
- Choline kinase inhibitors as a novel approach for antiproliferative drug design. | Hernández-Alcoceba, R., et al. 1997. Oncogene. 15: 2289-301. PMID: 9393874
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ChoK Antibody (B-8) | sc-376489 | 200 µg/ml | $316.00 | |||
ChoK Antibody (B-8): m-IgG Fc BP-HRP Bundle | sc-529879 | 200 µg Ab; 10 µg BP | $354.00 | |||
ChoK Antibody (B-8): m-IgGκ BP-HRP Bundle | sc-522972 | 200 µg Ab, 40 µg BP | $354.00 | |||
ChoK Antibody (B-8) AC | sc-376489 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ChoK Antibody (B-8) HRP | sc-376489 HRP | 200 µg/ml | $316.00 | |||
ChoK Antibody (B-8) FITC | sc-376489 FITC | 200 µg/ml | $330.00 | |||
ChoK Antibody (B-8) PE | sc-376489 PE | 200 µg/ml | $343.00 | |||
ChoK Antibody (B-8) Alexa Fluor® 488 | sc-376489 AF488 | 200 µg/ml | $357.00 | |||
ChoK Antibody (B-8) Alexa Fluor® 546 | sc-376489 AF546 | 200 µg/ml | $357.00 | |||
ChoK Antibody (B-8) Alexa Fluor® 594 | sc-376489 AF594 | 200 µg/ml | $357.00 | |||
ChoK Antibody (B-8) Alexa Fluor® 647 | sc-376489 AF647 | 200 µg/ml | $357.00 | |||
ChoK Antibody (B-8) Alexa Fluor® 680 | sc-376489 AF680 | 200 µg/ml | $357.00 | |||
ChoK Antibody (B-8) Alexa Fluor® 790 | sc-376489 AF790 | 200 µg/ml | $357.00 |