choactase Antibody (E-7): sc-55557

Choactase Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects choactase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-choactase antibody (E-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Choline acetyltransferase, also known as choactase, plays a critical role in synthesizing acetylcholine in cholinergic neurons, which is essential for neurotransmission and cognitive function. Choactase (E-7) antibody targets proteins primarily located in the cytoplasm, though the long isoform is found in cell nuclei, highlighting diverse functional roles depending on localization. The presence of multiple choactase mRNAs, such as R-, N1, N2-, S-, and M-types, allows for the production of different enzyme isoforms, which can influence the regulation of acetylcholine synthesis. Expression of choactase is intricately linked to neurodegenerative conditions, as decreased levels and increased activity of the NFkB pathway have been associated with aging and Alzheimer′s disease. This suggests that regulation of choactase expression is crucial for maintaining cholinergic function, and understanding cellular localization and isoform distribution could provide insights into therapeutic strategies for cognitive decline. Phosphorylation at Serine 440 has been identified as a key post-translational modification that enhances choactase′s catalytic activity, further emphasizing the importance in neuronal health and signaling.

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choactase Antibody (E-7) References:

1. Nuclear localization of the 82-kDa form of human choline acetyltransferase.  |  Resendes, MC., et al. 1999. J Biol Chem. 274: 19417-21. PMID: 10383456
2. Expression, purification and characterization of recombinant human choline acetyltransferase: phosphorylation of the enzyme regulates catalytic activity.  |  Dobransky, T., et al. 2000. Biochem J. 349: 141-51. PMID: 10861222
3. Nuclear factor kappaB/p49 is a negative regulatory factor in nerve growth factor-induced choline acetyltransferase promoter activity in PC12 cells.  |  Toliver-Kinsky, T., et al. 2000. J Neurochem. 75: 2241-51. PMID: 11080175
4. Functional characterization of phosphorylation of 69-kDa human choline acetyltransferase at serine 440 by protein kinase C.  |  Dobransky, T., et al. 2001. J Biol Chem. 276: 22244-50. PMID: 11303024
5. A complementary DNA for human choline acetyltransferase induces two forms of enzyme with different molecular weights in cultured cells.  |  Oda, Y., et al. 1992. Brain Res Mol Brain Res. 16: 287-94. PMID: 1337937
6. Decreased choline acetyltransferase mRNA expression in the nucleus basalis of Meynert in Alzheimer disease: an in situ hybridization study.  |  Strada, O., et al. 1992. Proc Natl Acad Sci U S A. 89: 9549-53. PMID: 1409664
7. Differentiation of neuronal stem cells into motor neurons using electrospun poly-L-lactic acid/gelatin scaffold.  |  Binan, L., et al. 2014. Biomaterials. 35: 664-74. PMID: 24161168
8. Immunohistochemical demonstration of choline acetyltransferase-immunoreactive preganglionic nerve fibers in guinea pig autonomic ganglia.  |  Lindh, B., et al. 1986. Proc Natl Acad Sci U S A. 83: 5316-20. PMID: 3460095
9. Streptozotocin-induced type 1 and 2 diabetes in rodents: a model for studying diabetic cardiac autonomic neuropathy.  |  Akinlade, OM., et al. 2021. Afr Health Sci. 21: 719-727. PMID: 34795728
10. cDNA cloning and complete sequence of porcine choline acetyltransferase: in vitro translation of the corresponding RNA yields an active protein.  |  Berrard, S., et al. 1987. Proc Natl Acad Sci U S A. 84: 9280-4. PMID: 3480542
11. Synthesizing enzymes for four neuroactive substances in motor neurons and neuromuscular junctions: light and electron microscopic immunocytochemistry.  |  Chan-Palay, V., et al. 1982. Proc Natl Acad Sci U S A. 79: 6717-21. PMID: 6128735
12. Human choline acetyltransferase mRNAs with different 5'-region produce a 69-kDa major translation product.  |  Misawa, H., et al. 1997. Brain Res Mol Brain Res. 44: 323-33. PMID: 9073174

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-choactase Antibody (E-7) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-choactase Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

choactase Antibody (E-7) Specifications

Alternate Product Name: Choline Acetyltransferase

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 561-655

Epitope Target: choactase

Epitope Species: human

Product Reactivity/Detection: all isoforms of choactase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for choactase Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin