Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CHMP6 CRISPR/Cas9 KO Plasmid (h): sc-406179
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CHMP6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CHMP6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CHMP6 Antibody (B-3): sc-398963
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHMP6 CRISPR/Cas9 KO Plasmid (h) | sc-406179 | 20 µg | $397.00 |
Overview
CHMP6 (charged multivesicular body protein 6) is a core component of the ESCRT-III machinery that coordinates endosomal membrane remodeling and multivesicular body biogenesis, enabling cargo sorting and lysosomal degradation. Through ESCRT-dependent membrane scission, CHMP6 helps regulate receptor downmodulation, exosome-related trafficking, and broader proteostasis and signaling homeostasis. ESCRT pathway perturbations have been linked to defects in endolysosomal trafficking, cytokinesis, autophagy, and viral budding, processes frequently altered in cancer and neurodegeneration. As a result, CHMP6 is routinely studied for its contribution to vesicle trafficking phenotypes, receptor signaling dynamics, and stress responses tied to endosomal dysfunction.
CHMP6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CHMP6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CHMP6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CHMP6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CHMP6 protein expression.
This CRISPR knockout system enables efficient generation of CHMP6-deficient cell models for investigation of CHMP6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CHMP6 exon(s) critical for CHMP6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CHMP6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CHMP6 CRISPR/Cas9 KO Plasmid (h) and CHMP6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CHMP6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CHMP6 HDR Plasmid (h) and CHMP6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CHMP6 homology arms to support homology-directed repair at defined CHMP6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.