CHMP4A Antibody (E-6): sc-514869
- CHMP4A Antibody (E-6) is a mouse monoclonal IgG2a κ CHMP4A antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 171-222 mapping at the C-terminus of CHMP4A of human origin
- CHMP4A Antibody (E-6) is recommended for detection of CHMP4A of human origin by WB, IP, IF and ELISA
- Anti-CHMP4A Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for CHMP4A Antibody (E-6) for WB applications. These reagents are now offered in bundles with CHMP4A Antibody (E-6) (see ordering information below).
CHMP4A Antibody (E-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects CHMP4A protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CHMP4A antibody (E-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CHMP4A protein is a member of the charged multivesicular body protein family, which plays a critical role in the endosomal sorting complex required for transport (ESCRT) pathway. This pathway is essential for the formation of multivesicular bodies (MVBs), which are involved in the degradation of membrane proteins and the sorting of proteins for exocytosis. CHMP4A, along with isoforms CHMP4B and CHMP4C, is particularly important during viral infections, such as HIV-1, where CHMP4A facilitates the budding and release of viral particles by interacting with viral proteins. CHMP4A expression is notably high in tissues such as the kidney, liver, heart, and skeletal muscle, indicating a vital role in cellular processes across various organ systems. Understanding CHMP4A function and interactions is crucial for elucidating involvement in both normal cellular physiology and disease states, making CHMP4A (E-6) monoclonal antibody an invaluable tool for researchers studying these processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CHMP4A Antibody (E-6) References:
- The protein network of HIV budding. | von Schwedler, UK., et al. 2003. Cell. 114: 701-13. PMID: 14505570
- The penta-EF-hand protein ALG-2 interacts directly with the ESCRT-I component TSG101, and Ca2+-dependently co-localizes to aberrant endosomes with dominant-negative AAA ATPase SKD1/Vps4B. | Katoh, K., et al. 2005. Biochem J. 391: 677-85. PMID: 16004603
- CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway. | Horii, M., et al. 2006. Biochem J. 400: 23-32. PMID: 16856878
- CHMP4b is a major binding partner of the ALG-2-interacting protein Alix among the three CHMP4 isoforms. | Katoh, K., et al. 2004. Arch Biochem Biophys. 421: 159-65. PMID: 14678797
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHMP4A Antibody (E-6) | sc-514869 | 200 µg/ml | $322.00 | |||
CHMP4A Antibody (E-6): m-IgG Fc BP-HRP Bundle | sc-526397 | 200 µg Ab; 10 µg BP | $361.00 | |||
CHMP4A Antibody (E-6): m-IgG2a BP-HRP Bundle | sc-546621 | 200 µg Ab; 10 µg BP | $361.00 | |||
CHMP4A Antibody (E-6) AC | sc-514869 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CHMP4A Antibody (E-6) HRP | sc-514869 HRP | 200 µg/ml | $322.00 | |||
CHMP4A Antibody (E-6) FITC | sc-514869 FITC | 200 µg/ml | $336.00 | |||
CHMP4A Antibody (E-6) PE | sc-514869 PE | 200 µg/ml | $349.00 | |||
CHMP4A Antibody (E-6) Alexa Fluor® 488 | sc-514869 AF488 | 200 µg/ml | $364.00 | |||
CHMP4A Antibody (E-6) Alexa Fluor® 546 | sc-514869 AF546 | 200 µg/ml | $364.00 | |||
CHMP4A Antibody (E-6) Alexa Fluor® 594 | sc-514869 AF594 | 200 µg/ml | $364.00 | |||
CHMP4A Antibody (E-6) Alexa Fluor® 647 | sc-514869 AF647 | 200 µg/ml | $364.00 | |||
CHMP4A Antibody (E-6) Alexa Fluor® 680 | sc-514869 AF680 | 200 µg/ml | $364.00 | |||
CHMP4A Antibody (E-6) Alexa Fluor® 790 | sc-514869 AF790 | 200 µg/ml | $364.00 |