ChM-1 Antibody (H-10): sc-365693
- ChM-1 Antibody (H-10) is a mouse monoclonal IgG2b κ ChM-1 antibody provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of ChM-1 of human origin
- ChM-1 Antibody (H-10) is recommended for detection of ChM-1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-ChM-1 Antibody (H-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for ChM-1 Antibody (H-10) for WB applications. This reagent is now offered in a bundle with ChM-1 Antibody (H-10) (see ordering information below).
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ChM-1 Antibody (H-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects ChM-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ChM-1 (H-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ChM-1 is a cartilage-specific matrix glycoprotein that plays a crucial role in stimulating chondrocyte growth, essential for cartilage development and maintenance. ChM-1 inhibits angiogenesis by disrupting endothelial cell tube formation, contributing to cartilage′s avascular nature. ChM-1 is predominantly expressed in proliferating and hypertrophic zones of epiphyseal plate in long bones, accumulating in interterritorial matrix surrounding lacunae. During development, ChM-1 downregulation allows angiogenesis and subsequent bone formation on cartilage template. ChM-1 expression is modulated by several growth factors, including transforming growth factor beta 2, fibroblast growth factor 2, and parathyroid hormone-like hormone, influencing cartilage and bone dynamics. Human ChM-1 gene is located on chromosome 13q14-q21, highlighting significance in developmental biology and potential pathological conditions such as pleomorphic adenomas, where altered ChM-1 expression may contribute to hypovascularity and chondroid formation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ChM-1 Antibody (H-10) References:
- Spatiotemporal pattern of the mouse chondromodulin-I gene expression and its regulatory role in vascular invasion into cartilage during endochondral bone formation. | Shukunami, C., et al. 1999. Int J Dev Biol. 43: 39-49. PMID: 10213081
- Genomic organization of the human chondromodulin-1 gene containing a promoter region that confers the expression of reporter gene in chondrogenic ATDC5 cells. | Yanagihara, I., et al. 2000. J Bone Miner Res. 15: 421-9. PMID: 10750556
- Cartilage-specific matrix protein chondromodulin-I is associated with chondroid formation in salivary pleomorphic adenomas: immunohistochemical analysis. | Kusafuka, K., et al. 2001. Am J Pathol. 158: 1465-72. PMID: 11290564
- [Differentiation of cartilage stem cells and their clinical applications]. | Shukunami, C. and Hiraki, Y. 2002. Clin Calcium. 12: 194-9. PMID: 15775294
- [Cell-cell and cell-matrix interactions during cartilage formation]. | Hiraki, Y. 2005. Fukuoka Igaku Zasshi. 96: 353-62. PMID: 16408492
- Molecular cloning of a new class of cartilage-specific matrix, chondromodulin-I, which stimulates growth of cultured chondrocytes. | Hiraki, Y., et al. 1991. Biochem Biophys Res Commun. 175: 971-7. PMID: 1709014
- CHM-1, a new vascular targeting agent, induces apoptosis of human umbilical vein endothelial cells via p53-mediated death receptor 5 up-regulation. | Tsai, AC., et al. 2010. J Biol Chem. 285: 5497-506. PMID: 20007968
- Chm-1 gene-modified bone marrow mesenchymal stem cells maintain the chondrogenic phenotype of tissue-engineered cartilage. | Chen, Z., et al. 2016. Stem Cell Res Ther. 7: 70. PMID: 27150539
- CHM-1, a novel microtubule-destabilizing agent exhibits antitumor activity via inducing the expression of SIRT2 in human breast cancer cells. | Liu, CW., et al. 2018. Chem Biol Interact. 289: 98-108. PMID: 29679549
- Inhibition of DNA synthesis and tube morphogenesis of cultured vascular endothelial cells by chondromodulin-I. | Hiraki, Y., et al. 1997. FEBS Lett. 415: 321-4. PMID: 9357992
- Identification of chondromodulin I as a novel endothelial cell growth inhibitor. Purification and its localization in the avascular zone of epiphyseal cartilage. | Hiraki, Y., et al. 1997. J Biol Chem. 272: 32419-26. PMID: 9405451
- Expression of cartilage-specific functional matrix chondromodulin-I mRNA in rabbit growth plate chondrocytes and its responsiveness to growth stimuli in vitro. | Shukunami, C. and Hiraki, Y. 1998. Biochem Biophys Res Commun. 249: 885-90. PMID: 9731231
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ChM-1 Antibody (H-10) | sc-365693 | 200 µg/ml | $322.00 | |||
ChM-1 Antibody (H-10): m-IgG2b BP-HRP Bundle | sc-548726 | 200 µg Ab; 10 µg BP | $361.00 | |||
ChM-1 Antibody (H-10) AC | sc-365693 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ChM-1 Antibody (H-10) HRP | sc-365693 HRP | 200 µg/ml | $322.00 | |||
ChM-1 Antibody (H-10) FITC | sc-365693 FITC | 200 µg/ml | $336.00 | |||
ChM-1 Antibody (H-10) PE | sc-365693 PE | 200 µg/ml | $349.00 | |||
ChM-1 Antibody (H-10) Alexa Fluor® 488 | sc-365693 AF488 | 200 µg/ml | $364.00 | |||
ChM-1 Antibody (H-10) Alexa Fluor® 546 | sc-365693 AF546 | 200 µg/ml | $364.00 | |||
ChM-1 Antibody (H-10) Alexa Fluor® 594 | sc-365693 AF594 | 200 µg/ml | $364.00 | |||
ChM-1 Antibody (H-10) Alexa Fluor® 647 | sc-365693 AF647 | 200 µg/ml | $364.00 | |||
ChM-1 Antibody (H-10) Alexa Fluor® 680 | sc-365693 AF680 | 200 µg/ml | $364.00 | |||
ChM-1 Antibody (H-10) Alexa Fluor® 790 | sc-365693 AF790 | 200 µg/ml | $364.00 |