CHIC1/2 Antibody (B-11): sc-515175
- CHIC1/2 Antibody (B-11) is a mouse monoclonal IgG1 κ CHIC1/2 antibody provided at 200 µg/ml
- raised against amino acids 1-165 representing full length CHIC2 of human origin
- CHIC1/2 Antibody (B-11) is recommended for detection of CHIC1 and CHIC2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CHIC1/2 Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CHIC1/2 Antibody (B-11) for WB applications. These reagents are now offered in bundles with CHIC1/2 Antibody (B-11) (see ordering information below).
CHIC1/2 Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects CHIC1/2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CHIC1/2 (B-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CHIC2, also known as Cysteine-rich hydrophobic domain 2 protein, plays a crucial role in cellular processes by regulating exocytosis, which is essential for secretion of hormones, neurotransmitters, and other important molecules. This 165 amino acid membrane protein is primarily located at plasma membrane and vesicular structures, where cysteine-rich hydrophobic motif undergoes palmitoylation, a post-translational modification necessary for membrane association. CHIC2 is implicated in chromosomal translocations associated with acute myeloid leukemias, particularly t(4;12)(q11;p13) translocation, which leads to formation of a fusion protein with TEL gene, resulting in altered gene expression and cellular behavior. Additionally, in systemic mast cell disease with eosinophilia, deletions of CHIC2 can occur, leading to FIP1L1-PDGFR-α rearrangement, which produces a constitutively active PDGFR-α, further highlighting CHIC2′s importance in both normal physiology and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CHIC1/2 Antibody (B-11) References:
- Fusion of a novel gene, BTL, to ETV6 in acute myeloid leukemias with a t(4;12)(q11-q12;p13). | Cools, J., et al. 1999. Blood. 94: 1820-4. PMID: 10477709
- A new family of small, palmitoylated, membrane-associated proteins, characterized by the presence of a cysteine-rich hydrophobic motif. | Cools, J., et al. 2001. FEBS Lett. 492: 204-9. PMID: 11257495
- CHIC2 deletion, a surrogate for FIP1L1-PDGFRA fusion, occurs in systemic mastocytosis associated with eosinophilia and predicts response to imatinib mesylate therapy. | Pardanani, A., et al. 2003. Blood. 102: 3093-6. PMID: 12842979
- A rare case of acute myeloid leukemia with a CHIC2-ETV6 fusiongen and multiple other molecular aberrations. | Kuchenbauer, F., et al. 2005. Leukemia. 19: 2366-8. PMID: 16224488
- Characterization of the amplicon on chromosomal segment 4q12 in glioblastoma multiforme. | Holtkamp, N., et al. 2007. Neuro Oncol. 9: 291-7. PMID: 17504929
- Validation of a new three-color fluorescence in situ hybridization (FISH) method to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocations. | Fink, SR., et al. 2009. Leuk Res. 33: 843-6. PMID: 19118897
- Analysis of nitric oxide-stabilized mRNAs in human fibroblasts reveals HuR-dependent heme oxygenase 1 upregulation. | Kuwano, Y., et al. 2009. Mol Cell Biol. 29: 2622-35. PMID: 19289500
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHIC1/2 Antibody (B-11) | sc-515175 | 200 µg/ml | $316.00 | |||
CHIC1/2 Antibody (B-11): m-IgG Fc BP-HRP Bundle | sc-531303 | 200 µg Ab; 10 µg BP | $354.00 | |||
CHIC1/2 Antibody (B-11): m-IgGκ BP-HRP Bundle | sc-524961 | 200 µg Ab, 40 µg BP | $354.00 | |||
CHIC1/2 Antibody (B-11): m-IgG1 BP-HRP Bundle | sc-544474 | 200 µg Ab; 20 µg BP | $354.00 | |||
CHIC1/2 Antibody (B-11) AC | sc-515175 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CHIC1/2 Antibody (B-11) HRP | sc-515175 HRP | 200 µg/ml | $316.00 | |||
CHIC1/2 Antibody (B-11) FITC | sc-515175 FITC | 200 µg/ml | $330.00 | |||
CHIC1/2 Antibody (B-11) PE | sc-515175 PE | 200 µg/ml | $343.00 | |||
CHIC1/2 Antibody (B-11) Alexa Fluor® 488 | sc-515175 AF488 | 200 µg/ml | $357.00 | |||
CHIC1/2 Antibody (B-11) Alexa Fluor® 546 | sc-515175 AF546 | 200 µg/ml | $357.00 | |||
CHIC1/2 Antibody (B-11) Alexa Fluor® 594 | sc-515175 AF594 | 200 µg/ml | $357.00 | |||
CHIC1/2 Antibody (B-11) Alexa Fluor® 647 | sc-515175 AF647 | 200 µg/ml | $357.00 | |||
CHIC1/2 Antibody (B-11) Alexa Fluor® 680 | sc-515175 AF680 | 200 µg/ml | $357.00 | |||
CHIC1/2 Antibody (B-11) Alexa Fluor® 790 | sc-515175 AF790 | 200 µg/ml | $357.00 |