CHD6 Antibody (E-6) recommended for detection of CHD6 of mouse, rat and human origin by WB, IP, IF and ELISA

CHD6 Antibody (E-6): sc-393445

CHD6 Antibody (E-6) is rated 3.0 out of 5 by 2.
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Datasheets

    • Anti-CHD6 Antibody (E-6) is a mouse monoclonal IgG2a (kappa light chain) CHD6 antibody provided at 200 µg/ml
    • raised against amino acids 243-320 mapping near the N-terminus of CHD6 of human origin
    • Anti-CHD6 Antibody (E-6) is recommended for detection of CHD6 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-CHD6 Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-393445 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CHD6 (E-6): sc-393445.

What blocking solution should I use with CHD6 (E-6): sc-393445 monoclonal antibody for Western blot?

Asked by: Germaine
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 1 out of 5 by from sc-393445 does not work with IFs We tried the sc-393445 anti-CHD6 in immunofluorescent antibodies and we could not detect it under the microscope. Used on crosslinked HUVECs with 4% paraformaldehyde
Date published: 2017-12-18
Rated 5 out of 5 by from Very good Western blot data of CHD6 expression Very good Western blot data of CHD6 expression in HeLa and Y79 whole cell lysates. -SCBT QC
Date published: 2013-10-01
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