Ceramide Kinase Antibody (G-3): sc-376730

Ceramide Kinase Antibody (G-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects Ceramide Kinase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Ceramide Kinase antibody (G-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ceramide Kinase, also known as CERK, LK4 (lipid kinase 4), or acylsphingosine kinase, is a 537 amino acid peripheral membrane protein primarily localized to membrane and cytoplasm. Such localization enables Ceramide Kinase to effectively interact with substrates and participate in key signaling pathways. Highly expressed in brain, liver, kidney, heart, and skeletal muscle, with lower expression levels in spleen, lung, thymus, and small intestine, Ceramide Kinase utilizes calcium and magnesium as cofactors to catalyze ATP-dependent conversion of ceramide to ceramide 1-phosphate (C1P), a significant sphingolipid metabolite. Operating optimally at a pH range of 6-7.5, Ceramide Kinase plays a vital role in various cellular processes, including apoptosis, phagocytosis, and cellular proliferation, highlighting its importance in maintaining cellular homeostasis and responding to stress signals.

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Ceramide Kinase Antibody (G-3) References:

1. Ceramide kinase, a novel lipid kinase. Molecular cloning and functional characterization.  |  Sugiura, M., et al. 2002. J Biol Chem. 277: 23294-300. PMID: 11956206
2. Ceramide 1-phosphate, a mediator of phagocytosis.  |  Hinkovska-Galcheva, V., et al. 2005. J Biol Chem. 280: 26612-21. PMID: 15899891
3. Substrate specificity of human ceramide kinase.  |  Wijesinghe, DS., et al. 2005. J Lipid Res. 46: 2706-16. PMID: 16170208
4. Further characterization of mammalian ceramide kinase: substrate delivery and (stereo)specificity, tissue distribution, and subcellular localization studies.  |  Van Overloop, H., et al. 2006. J Lipid Res. 47: 268-83. PMID: 16269826
5. Ceramide kinase regulates growth and survival of A549 human lung adenocarcinoma cells.  |  Mitra, P., et al. 2007. FEBS Lett. 581: 735-40. PMID: 17274985
6. Ceramide kinase uses ceramide provided by ceramide transport protein: localization to organelles of eicosanoid synthesis.  |  Lamour, NF., et al. 2007. J Lipid Res. 48: 1293-304. PMID: 17392267
7. Ceramide kinase and ceramide-1-phosphate.  |  Wijesinghe, DS., et al. 2007. Methods Enzymol. 434: 265-92. PMID: 17954253
8. Ceramide kinase expression is altered during macrophage-like cell differentiation of the leukemia cell line HL-60.  |  Date, T., et al. 2007. In Vitro Cell Dev Biol Anim. 43: 321-3. PMID: 17957442
9. Ceramide kinase promotes Ca2+ signaling near IgG-opsonized targets and enhances phagolysosomal fusion in COS-1 cells.  |  Hinkovska-Galcheva, V., et al. 2008. J Lipid Res. 49: 531-42. PMID: 18156590
10. Implication of Ceramide Kinase/C1P in Cancer Development and Progression.  |  Camacho, L., et al. 2022. Cancers (Basel). 14: PMID: 35008391
11. Ceramide Kinase Inhibition Drives Ferroptosis and Sensitivity to Cisplatin in Mutant KRAS Lung Cancer by Dysregulating VDAC-Mediated Mitochondria Function.  |  Vu, NT., et al. 2022. Mol Cancer Res. 20: 1429-1442. PMID: 35560154
12. Ceramide kinase-mediated C1P metabolism attenuates acute liver injury by inhibiting the interaction between KEAP1 and NRF2.  |  Dong, W., et al. 2024. Exp Mol Med. 56: 946-958. PMID: 38556546

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ceramide Kinase Antibody (G-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Ceramide Kinase Antibody (G-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ceramide Kinase Antibody (G-3) Specifications

Alternate Product Name: Ceramide Kinase

Product Clone Name: G-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 238-474

Epitope Target: Ceramide Kinase

Epitope Species: human

Product Reactivity/Detection: Ceramide Kinase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Ceramide Kinase Antibody (G-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin