Centriolin Antibody (C-9): sc-365521
- Centriolin Antibody (C-9) is a mouse monoclonal IgG1 κ Centriolin antibody, cited in 18 publications, provided at 200 µg/ml
- raised against amino acids 2026-2325 mapping at the C-terminus of Centriolin of human origin
- Centriolin Antibody (C-9) is recommended for detection of Centriolin of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Centriolin Antibody (C-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Centriolin Antibody (C-9) for WB and IHC(P) applications. These reagents are now offered in bundles with Centriolin Antibody (C-9) (see ordering information below).
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Centriolin Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Centriolin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Centriolin antibody (C-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Centriolin, also known as CEP110, is a coiled-coil centrosomal protein that plays a crucial role in centrosome maturation and function, particularly during late stages of mitosis when cytokinesis occurs. This process is essential for successful division of cytoplasm, resulting in two daughter cells. Centriolin is strategically located at midbody during abscission, final step of cytokinesis, where Centriolin serves as an anchor for vesicle-fusion and exocyst complexes, facilitating separation of two cells. By regulating initiation of this critical step, Centriolin not only ensures proper cell division but also influences cell proliferation and transition into S phase of mitosis. Centriolin′s ability to coordinate these processes underscores its importance in maintaining cellular integrity and function, making Centriolin a vital target for research in cell biology and cancer studies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Centriolin Antibody (C-9) References:
- FGFR1 is fused to the centrosome-associated protein CEP110 in the 8p12 stem cell myeloproliferative disorder with t(8;9)(p12;q33). | Guasch, G., et al. 2000. Blood. 95: 1788-96. PMID: 10688839
- CEP110 and ninein are located in a specific domain of the centrosome associated with centrosome maturation. | Ou, YY., et al. 2002. J Cell Sci. 115: 1825-35. PMID: 11956314
- A novel human protein of the maternal centriole is required for the final stages of cytokinesis and entry into S phase. | Gromley, A., et al. 2003. J Cell Biol. 161: 535-45. PMID: 12732615
- Humoral detection of leukaemia-associated antigens in presentation acute myeloid leukaemia. | Guinn, BA., et al. 2005. Biochem Biophys Res Commun. 335: 1293-304. PMID: 16112646
- Centriolin anchoring of exocyst and SNARE complexes at the midbody is required for secretory-vesicle-mediated abscission. | Gromley, A., et al. 2005. Cell. 123: 75-87. PMID: 16213214
- A ring-like template for abscission. | von Dassow, G. and Bement, WM. 2005. Dev Cell. 9: 578-80. PMID: 16256733
- Hook2 localizes to the centrosome, binds directly to centriolin/CEP110 and contributes to centrosomal function. | Szebenyi, G., et al. 2007. Traffic. 8: 32-46. PMID: 17140400
- Hook2 contributes to aggresome formation. | Szebenyi, G., et al. 2007. BMC Cell Biol. 8: 19. PMID: 17540036
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Centriolin Antibody (C-9) | sc-365521 | 200 µg/ml | $322.00 | |||
Centriolin Antibody (C-9): m-IgG Fc BP-HRP Bundle | sc-529386 | 200 µg Ab; 10 µg BP | $361.00 | |||
Centriolin Antibody (C-9): m-IgGκ BP-HRP Bundle | sc-522263 | 200 µg Ab, 40 µg BP | $361.00 | |||
Centriolin Antibody (C-9): m-IgG1 BP-HRP Bundle | sc-543493 | 200 µg Ab; 20 µg BP | $361.00 | |||
Centriolin Antibody (C-9) AC | sc-365521 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Centriolin Antibody (C-9) HRP | sc-365521 HRP | 200 µg/ml | $322.00 | |||
Centriolin Antibody (C-9) FITC | sc-365521 FITC | 200 µg/ml | $336.00 | |||
Centriolin Antibody (C-9) PE | sc-365521 PE | 200 µg/ml | $349.00 | |||
Centriolin Antibody (C-9) Alexa Fluor® 488 | sc-365521 AF488 | 200 µg/ml | $364.00 | |||
Centriolin Antibody (C-9) Alexa Fluor® 546 | sc-365521 AF546 | 200 µg/ml | $364.00 | |||
Centriolin Antibody (C-9) Alexa Fluor® 594 | sc-365521 AF594 | 200 µg/ml | $364.00 | |||
Centriolin Antibody (C-9) Alexa Fluor® 647 | sc-365521 AF647 | 200 µg/ml | $364.00 | |||
Centriolin Antibody (C-9) Alexa Fluor® 680 | sc-365521 AF680 | 200 µg/ml | $364.00 | |||
Centriolin Antibody (C-9) Alexa Fluor® 790 | sc-365521 AF790 | 200 µg/ml | $364.00 |