Centrin-2 CRISPR/Cas9 KO Plasmid (h): sc-400867
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Centrin-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Centrin-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Centrin-2 Antibody (3F8): sc-293192
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Centrin-2 CRISPR/Cas9 KO Plasmid (h) | sc-400867 | 20 µg | $397.00 |
Overview
CETN2 encodes centrin-2, a conserved EF-hand calcium-binding protein that localizes to centrosomes and basal bodies and contributes to centriole duplication, centrosome integrity, and spindle organization during cell division. Centrin-2 also participates in primary cilium assembly and function, linking CETN2 activity to cell-cycle control and microtubule-based processes such as ciliogenesis. Through roles in centrosome homeostasis and ciliary signaling networks, altered centrin-2 dynamics can influence genomic stability and cellular differentiation programs that are frequently perturbed in cancer and ciliopathy-related biology. CETN2 is therefore widely used as a molecular handle to interrogate centrosome-associated pathways, mitotic fidelity, and cilia-dependent signaling outputs in human cells.
Centrin-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CETN2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CETN2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CETN2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Centrin-2 protein expression.
This CRISPR knockout system enables efficient generation of CETN2-deficient cell models for investigation of Centrin-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CETN2 exon(s) critical for Centrin-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CETN2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Centrin-2 CRISPR/Cas9 KO Plasmid (h) and Centrin-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CETN2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Centrin-2 HDR Plasmid (h) and Centrin-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CETN2 homology arms to support homology-directed repair at defined CETN2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.