Centrin-2 Antibody (3F8): sc-293192
- Centrin-2 Antibody (3F8) is a mouse monoclonal IgG1 κ Centrin-2 antibody, cited in 1 publications, provided at 100 µg/ml
- raised against amino acids 85-172 of Centrin-2 of human origin
- recommended for detection of Centrin-2 of mouse, rat and human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Centrin-2 Antibody (3F8). This work is in progress.
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Centrin-2 Antibody (3F8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Centrin-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Centrin-2 antibody (3F8) is available as a non-conjugated format. Centrin-2, an EF-hand protein, is essential for normal cell division and is predominantly located in the centrosomes, mitotic spindle poles, and basal bodies of cells. Expression in tissues with cilia, such as the retina and testis, highlights Centrin-2′s importance in cellular functions related to motility and signaling. Centrin-2 is also expressed in non-differentiated, non-ciliated retinal cells, such as retinoblastoma cells, as well as in liver, skeletal muscle, and cardiac muscle. Centrin-2′s role extends beyond cell division; Centrin-2 is involved in the organization of spindle pole morphology and the completion of cytokinesis, as evidenced by knockdown studies that demonstrate a requirement for Centrin-2 in centriole duplication. Additionally, Centrin-2 is implicated in nucleotide excision repair through association with the xeroderma pigmentosum group C protein, suggesting a critical link between cell division and DNA repair mechanisms. This multifaceted role underscores Centrin-2′s significance in maintaining cellular integrity and function across various tissues.
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Centrin-2 Antibody (3F8) References:
- Cation- and peptide-binding properties of human centrin 2. | Durussel, I., et al. 2000. FEBS Lett. 472: 208-12. PMID: 10788612
- Centrosome protein centrin 2/caltractin 1 is part of the xeroderma pigmentosum group C complex that initiates global genome nucleotide excision repair. | Araki, M., et al. 2001. J Biol Chem. 276: 18665-72. PMID: 11279143
- Centrin-2 is required for centriole duplication in mammalian cells. | Salisbury, JL., et al. 2002. Curr Biol. 12: 1287-92. PMID: 12176356
- Centriole duplication: centrin in on answers? | Rice, LM. and Agard, DA. 2002. Curr Biol. 12: R618-9. PMID: 12372266
- C-terminal half of human centrin 2 behaves like a regulatory EF-hand domain. | Matei, E., et al. 2003. Biochemistry. 42: 1439-50. PMID: 12578356
- SUMO-dependent regulation of centrin-2. | Klein, UR. and Nigg, EA. 2009. J Cell Sci. 122: 3312-21. PMID: 19706679
- CDC25B overexpression stabilises centrin 2 and promotes the formation of excess centriolar foci. | Boutros, R., et al. 2013. PLoS One. 8: e67822. PMID: 23840880
- Calcium-binding capacity of centrin2 is required for linear POC5 assembly but not for nucleotide excision repair. | Dantas, TJ., et al. 2013. PLoS One. 8: e68487. PMID: 23844208
- Phosphorylation promotes the endonuclease-like activity of human centrin 2. | Yang, J., et al. 2022. RSC Adv. 12: 21892-21903. PMID: 36043059
- Astrocytic centrin-2 expression in entorhinal cortex correlates with Alzheimer's disease severity. | Degl'Innocenti, E., et al. 2024. Glia. 72: 2158-2177. PMID: 39145525
- Expression of centrin isoforms in the mammalian retina. | Wolfrum, U. and Salisbury, JL. 1998. Exp Cell Res. 242: 10-7. PMID: 9665797
- Differential regulation of centrin genes during ciliogenesis in human tracheal epithelial cells. | LeDizet, M., et al. 1998. Am J Physiol. 275: L1145-56. PMID: 9843852
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Centrin-2 Antibody (3F8) | sc-293192 | 100 µg/ml | $316.00 |