Centaurin α2 Antibody (F-4): sc-514510
- Centaurin α2 Antibody (F-4) is a mouse monoclonal IgG1 κ Centaurin α2 antibody provided at 200 µg/ml
- raised against amino acids 271-381 mapping at the C-terminus of Centaurin α2 of human origin
- Centaurin alpha 2 Antibody (F-4) is recommended for detection of Centaurin α2 of human origin by WB, IP, IF and ELISA
- Anti-Centaurin alpha 2 Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Centaurin α2 Antibody (F-4) for WB applications. This reagent is now offered in a bundle with Centaurin α2 Antibody (F-4) (see ordering information below).
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Centaurin α2 Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Centaurin alpha 2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Centaurin α2 (F-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Centaurin alpha 2 protein plays a crucial role in regulating vesicular transport and cytoskeletal organization, primarily through interactions with the ADP-ribosylation factor (ARF) family of small GTP-binding proteins. These proteins are essential for maintaining cellular functions such as membrane trafficking and cell adhesion. Centaurin α2 is part of the Centaurin GTPase-activating protein family, which is involved in transducing phosphoinositide 3-kinase activation into the regulation of ARF-dependent pathways. Notably, Centaurin α2 is expressed in various tissues, including adipose tissue, heart, and skeletal muscle, and negatively regulates ARF-mediated actin rearrangement by binding to activated phosphoinositide 3-kinase. While the precise function of Centaurin α2 remains to be fully elucidated, high sequence similarity to Centaurin α1 suggests a potential role as an ARF6 GTPase, highlighting significance in cellular signaling and structural dynamics.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Centaurin α2 Antibody (F-4) References:
- Casein kinase I associates with members of the centaurin-alpha family of phosphatidylinositol 3,4,5-trisphosphate-binding proteins. | Dubois, T., et al. 2001. J Biol Chem. 276: 18757-64. PMID: 11278595
- Identification of centaurin-alpha2: a phosphatidylinositide-binding protein present in fat, heart and skeletal muscle. | Whitley, P., et al. 2002. Eur J Cell Biol. 81: 222-30. PMID: 12018390
- Cytohesins and centaurins control subcellular trafficking of macromolecular signaling complexes: regulation by phosphoinositides and ADP-ribosylation factors. | Hawadle, MA., et al. 2002. Biol Res. 35: 247-65. PMID: 12415743
- Identification of gene structure and subcellular localization of human centaurin alpha 2, and p42IP4, a family of two highly homologous, Ins 1,3,4,5-P4-/PtdIns 3,4,5-P3-binding, adapter proteins. | Hanck, T., et al. 2004. J Neurochem. 88: 326-36. PMID: 14690521
- The arf6 GAP centaurin alpha-1 is a neuronal actin-binding protein which also functions via GAP-independent activity to regulate the actin cytoskeleton. | Thacker, E., et al. 2004. Eur J Cell Biol. 83: 541-54. PMID: 15679100
- Evidence by expression analysis of candidate genes for congenital heart defects in the NF1 microdeletion interval. | Venturin, M., et al. 2005. Ann Hum Genet. 69: 508-16. PMID: 16138909
- PI-3-kinase-dependent membrane recruitment of centaurin-alpha2 is essential for its effect on ARF6-mediated actin cytoskeleton reorganisation. | Venkateswarlu, K., et al. 2007. J Cell Sci. 120: 792-801. PMID: 17284522
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Centaurin α2 Antibody (F-4) | sc-514510 | 200 µg/ml | $316.00 | |||
Centaurin α2 Antibody (F-4): m-IgG Fc BP-HRP Bundle | sc-526375 | 200 µg Ab; 10 µg BP | $354.00 | |||
Centaurin α2 Antibody (F-4) AC | sc-514510 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Centaurin α2 Antibody (F-4) HRP | sc-514510 HRP | 200 µg/ml | $316.00 | |||
Centaurin α2 Antibody (F-4) FITC | sc-514510 FITC | 200 µg/ml | $330.00 | |||
Centaurin α2 Antibody (F-4) PE | sc-514510 PE | 200 µg/ml | $343.00 | |||
Centaurin α2 Antibody (F-4) Alexa Fluor® 488 | sc-514510 AF488 | 200 µg/ml | $357.00 | |||
Centaurin α2 Antibody (F-4) Alexa Fluor® 546 | sc-514510 AF546 | 200 µg/ml | $357.00 | |||
Centaurin α2 Antibody (F-4) Alexa Fluor® 594 | sc-514510 AF594 | 200 µg/ml | $357.00 | |||
Centaurin α2 Antibody (F-4) Alexa Fluor® 647 | sc-514510 AF647 | 200 µg/ml | $357.00 | |||
Centaurin α2 Antibody (F-4) Alexa Fluor® 680 | sc-514510 AF680 | 200 µg/ml | $357.00 | |||
Centaurin α2 Antibody (F-4) Alexa Fluor® 790 | sc-514510 AF790 | 200 µg/ml | $357.00 |