Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Centaurin α1 CRISPR/Cas9 KO Plasmid (h): sc-407463
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Centaurin α1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Centaurin α1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Centaurin α1 Antibody (G-4): sc-390498
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Centaurin α1 CRISPR/Cas9 KO Plasmid (h) | sc-407463 | 20 µg | $397.00 |
Overview
ADAP1 encodes centaurin α1, an Arf GTPase-activating protein with a pleckstrin homology domain that couples phosphoinositide signaling to membrane trafficking and actin cytoskeleton remodeling. By modulating Arf-dependent endocytic and vesicular transport events, centaurin α1 helps coordinate receptor recycling and signal propagation downstream of PI3K-generated lipids. The protein has been linked to regulation of cell migration and adhesion dynamics, processes central to tissue organization and cellular responsiveness. Altered ADAP1 activity has been investigated in contexts where dysregulated trafficking and cytoskeletal control contribute to disease-associated phenotypes, including invasive behavior and aberrant signaling.
Centaurin α1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ADAP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ADAP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ADAP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Centaurin α1 protein expression.
This CRISPR knockout system enables efficient generation of ADAP1-deficient cell models for investigation of Centaurin α1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ADAP1 exon(s) critical for Centaurin α1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ADAP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Centaurin α1 CRISPR/Cas9 KO Plasmid (h) and Centaurin α1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ADAP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Centaurin α1 HDR Plasmid (h) and Centaurin α1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ADAP1 homology arms to support homology-directed repair at defined ADAP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.