CENP-M Antibody (B-7): sc-398754
- CENP-M Antibody (B-7) is a mouse monoclonal IgG2a κ CENP-M antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1-27 at the N-terminus of CENP-M of human origin
- CENP-M Antibody (B-7) is recommended for detection of CENP-M of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CENP-M Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CENP-M Antibody (B-7) for WB applications. These reagents are now offered in bundles with CENP-M Antibody (B-7) (see ordering information below).
CENP-M Antibody (B-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects CENP-M of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-CENP-M antibody (B-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CENP-M, also known as centromere protein M, ICEN39, or PANE1, is a 180 amino acid protein that plays a pivotal role in the assembly of the kinetochore during mitosis, which is essential for accurate chromosome segregation. CENP-M is primarily localized in the nucleus of non-confluent cells and shifts to the cytoplasm in dividing or confluent cells, highlighting a dynamic role in cell division. CENP-M functions as a key component of the CENPA-NAC complex, which is crucial for the assembly and function of the centromere, a specialized chromatin structure that ensures proper chromosome alignment and segregation. CENP-M contributes to the incorporation of newly synthesized CENP-A into centromeres, further emphasizing an importance in maintaining genomic stability. Three isoforms of CENP-M exist due to alternative splicing, with isoform 3 being expressed in B-lineage chronic lymphocytic leukemia (B-CLL) cells, suggesting a potential link to cancer development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CENP-M Antibody (B-7) References:
- Identification of genes differentially expressed in mouse mammary epithelium transformed by an activated beta-catenin. | Renou, JP., et al. 2003. Oncogene. 22: 4594-610. PMID: 12881717
- The proliferation associated nuclear element (PANE1) is conserved between mammals and fish and preferentially expressed in activated lymphoid cells. | Bierie, B., et al. 2004. Gene Expr Patterns. 4: 389-95. PMID: 15183305
- The PANE1 gene encodes a novel human minor histocompatibility antigen that is selectively expressed in B-lymphoid cells and B-CLL. | Brickner, AG., et al. 2006. Blood. 107: 3779-86. PMID: 16391015
- The human CENP-A centromeric nucleosome-associated complex. | Foltz, DR., et al. 2006. Nat Cell Biol. 8: 458-69. PMID: 16622419
- The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres. | Okada, M., et al. 2006. Nat Cell Biol. 8: 446-57. PMID: 16622420
- Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells. | Izuta, H., et al. 2006. Genes Cells. 11: 673-84. PMID: 16716197
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CENP-M Antibody (B-7) | sc-398754 | 200 µg/ml | $316.00 | |||
CENP-M Antibody (B-7): m-IgG Fc BP-HRP Bundle | sc-530916 | 200 µg Ab; 10 µg BP | $354.00 | |||
CENP-M Antibody (B-7): m-IgGκ BP-HRP Bundle | sc-524470 | 200 µg Ab, 40 µg BP | $354.00 | |||
CENP-M Antibody (B-7): m-IgG2a BP-HRP Bundle | sc-547629 | 200 µg Ab; 10 µg BP | $354.00 | |||
CENP-M Antibody (B-7) AC | sc-398754 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CENP-M Antibody (B-7) HRP | sc-398754 HRP | 200 µg/ml | $316.00 | |||
CENP-M Antibody (B-7) FITC | sc-398754 FITC | 200 µg/ml | $330.00 | |||
CENP-M Antibody (B-7) PE | sc-398754 PE | 200 µg/ml | $343.00 | |||
CENP-M Antibody (B-7) Alexa Fluor® 488 | sc-398754 AF488 | 200 µg/ml | $357.00 | |||
CENP-M Antibody (B-7) Alexa Fluor® 546 | sc-398754 AF546 | 200 µg/ml | $357.00 | |||
CENP-M Antibody (B-7) Alexa Fluor® 594 | sc-398754 AF594 | 200 µg/ml | $357.00 | |||
CENP-M Antibody (B-7) Alexa Fluor® 647 | sc-398754 AF647 | 200 µg/ml | $357.00 | |||
CENP-M Antibody (B-7) Alexa Fluor® 680 | sc-398754 AF680 | 200 µg/ml | $357.00 | |||
CENP-M Antibody (B-7) Alexa Fluor® 790 | sc-398754 AF790 | 200 µg/ml | $357.00 | |||
CENP-M (B-7) Neutralizing Peptide | sc-398754 P | 100 µg/0.5 ml | $68.00 |